Ammonium phosphinate

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from peer reviewed publication

Data source

Reference

Reference Type:

publication

Title:

Eye irritation toxicity study of the test chemical

Author:

Weiner et al

Year:

2001

Bibliographic source:

Food and Chemical Toxicology

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Standard eye irritation protocols

Principles of method if other than guideline:

A Draize eye irritation test was carried out for test chemical to assess the degree of eye irritation caused by the chemical.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Ammonium dihydrogenorthophosphate

EC Number:

231-764-5

EC Name:

Ammonium dihydrogenorthophosphate

Cas Number:

7722-76-1

Molecular formula:

H3N.H3O4P

IUPAC Name:

Ammonium dihydrogenorthophosphate

Details on test material:

Name: Ammonium dihydrogenorthophosphate
Common Name: MAP
InChI: 1S/H3N.H3O4P/c;1-5(2,3)4/h1H3;(H3,1,2,3,4)
Smiles: P(O)(O)(=O)[O-].[NH4+]
Molecular formula: H6NO4P
Molecular weight:115.024 g/mol
Substance type:Inorganic

Test animals / tissue source

Species:

rabbit

Strain:

not specified

Details on test animals or tissues and environmental conditions:

not specified

Test system

Vehicle:

not specified

Controls:

yes

Amount / concentration applied:

100mg (0.1ml)

Duration of treatment / exposure:

24 hours

Observation period (in vivo):

24 /48/72 hours

Number of animals or in vitro replicates:

6 rabbits

Details on study design:

TEST SITE
- Area of exposure: conjunctival sac of one eye of six rabbits

REMOVAL OF TEST SUBSTANCE
- Washing (if done):
No data
- Time after start of exposure:
No data

SCORING SYSTEM: Scores were based on the degree or extent of opacity on the cornea, the redness on the iris and the chemosis and discharge on the conjunctiva, using a standard scale at intervals of 1, 24, 48 and 72 h or until all irritation resolves. More emphasis was placed on the opacity of the cornea, which was given a maximum score of 80. Conjunctival changes had a maximum score of 20 and iritis had maximum score of 10. The total maximum score was 110.

TOOL USED TO ASSESS SCORE: No detailed data is available

Results and discussion

In vivo

Results

Irritation parameter:

overall irritation score

Basis:

mean

Time point:

24 h

Max. score:

110

Reversibility:

not specified

Remarks on result:

positive indication of irritation

Irritant / corrosive response data:

Slight eye irritation was observed in treated rabbits.

Other effects:

No data

Applicant's summary and conclusion

Interpretation of results:

Category 2 (irritating to eyes) based on GHS criteria

Conclusions:

The test chemical was considered to be Slightly irritating to the eyes of six rabbits and hence it is likely to classify as an eye irritant.

Executive summary:

Draize eye irritation test was carried out for test chemical to assess the degree of eye irritation caused by the chemical. In this study, 100 mg test material was instilled into the conjunctival sac of one eye each of six rabbits. The other eye served as a control. The eyelid was held shut for a few seconds and released. Washing, when indicated, occurred within 20–30 s of exposure and consisted of flooding the eye with large amounts of water for 1 min. Scores were based on the degree or extent of opacity on the cornea, the redness on the iris and the chemosis and discharge on the conjunctiva, using a standard scale at intervals of 1, 24, 48 and 72 h or until all irritation resolves. More emphasis was placed on the opacity of the cornea, which was given a maximum score of 80. Conjunctival changes had a maximum score of 20 and iritis had maximum score of 10. The total maximum score was 110. The chemical induced slight eye irritation in treated rabbits after 24 hours. Hence the test chemical was considered to be slightly irritating to the eyes of six rabbits and hence it is likely to classify as an eye irritant.