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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
Data is from peer reviewed publication

Data source

Reference
Reference Type:
publication
Title:
Eye irritation toxicity study of the test chemical
Author:
Weiner et al
Year:
2001
Bibliographic source:
Food and Chemical Toxicology

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: Standard eye irritation protocols
Principles of method if other than guideline:
A Draize eye irritation test was carried out for test chemical to assess the degree of eye irritation caused by the chemical.
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Ammonium dihydrogenorthophosphate
EC Number:
231-764-5
EC Name:
Ammonium dihydrogenorthophosphate
Cas Number:
7722-76-1
Molecular formula:
H3N.H3O4P
IUPAC Name:
Ammonium dihydrogenorthophosphate
Details on test material:
Name: Ammonium dihydrogenorthophosphate
Common Name: MAP
InChI: 1S/H3N.H3O4P/c;1-5(2,3)4/h1H3;(H3,1,2,3,4)
Smiles: P(O)(O)(=O)[O-].[NH4+]
Molecular formula: H6NO4P
Molecular weight:115.024 g/mol
Substance type:Inorganic

Test animals / tissue source

Species:
rabbit
Strain:
not specified
Details on test animals or tissues and environmental conditions:
not specified

Test system

Vehicle:
not specified
Controls:
yes
Amount / concentration applied:
100mg (0.1ml)
Duration of treatment / exposure:
24 hours
Observation period (in vivo):
24 /48/72 hours
Number of animals or in vitro replicates:
6 rabbits
Details on study design:
TEST SITE
- Area of exposure: conjunctival sac of one eye of six rabbits

REMOVAL OF TEST SUBSTANCE
- Washing (if done):
No data
- Time after start of exposure:
No data

SCORING SYSTEM: Scores were based on the degree or extent of opacity on the cornea, the redness on the iris and the chemosis and discharge on the conjunctiva, using a standard scale at intervals of 1, 24, 48 and 72 h or until all irritation resolves. More emphasis was placed on the opacity of the cornea, which was given a maximum score of 80. Conjunctival changes had a maximum score of 20 and iritis had maximum score of 10. The total maximum score was 110.

TOOL USED TO ASSESS SCORE: No detailed data is available

Results and discussion

In vivo

Results
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
24 h
Max. score:
110
Reversibility:
not specified
Remarks on result:
positive indication of irritation
Irritant / corrosive response data:
Slight eye irritation was observed in treated rabbits.
Other effects:
No data

Applicant's summary and conclusion

Interpretation of results:
Category 2 (irritating to eyes) based on GHS criteria
Conclusions:
The test chemical was considered to be Slightly irritating to the eyes of six rabbits and hence it is likely to classify as an eye irritant.
Executive summary:

Draize eye irritation test was carried out for test chemical to assess the degree of eye irritation caused by the chemical. In this study, 100 mg test material was instilled into the conjunctival sac of one eye each of six rabbits. The other eye served as a control. The eyelid was held shut for a few seconds and released. Washing, when indicated, occurred within 20–30 s of exposure and consisted of flooding the eye with large amounts of water for 1 min. Scores were based on the degree or extent of opacity on the cornea, the redness on the iris and the chemosis and discharge on the conjunctiva, using a standard scale at intervals of 1, 24, 48 and 72 h or until all irritation resolves. More emphasis was placed on the opacity of the cornea, which was given a maximum score of 80. Conjunctival changes had a maximum score of 20 and iritis had maximum score of 10. The total maximum score was 110. The chemical induced slight eye irritation in treated rabbits after 24 hours. Hence the test chemical was considered to be slightly irritating to the eyes of six rabbits and hence it is likely to classify as an eye irritant.