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EC number: 204-977-6 | CAS number: 130-15-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Deviations:
- yes
- Remarks:
- minor pH change in controls not considered to have affected study integrity
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- IDENTIFICATION AND QUANTIFICATION OF TEST SUBSTANCE/PRODUCT
- Separation method (e.g. HPLC, GC): HPLC
- Conditions (column, mobile phase, etc.): Eclipse plus C18, 1.8 µm, 4.6 mm i.d. x 50 mm. Eluant was ultra pure water and acetonitrile.
- Detection method (e.g. ECD, UV, MS, ICP-AES, ICP-MS): Photodiode array detector
- Detection limits (LOD, LOQ) (indicate method of determination/calculation): LOD 0.001 mg/L - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution was initially prepared at 100 mg/L. This was stirred for 48 hours and then filtered. The resulting solution was then further diluted to provide the test substance concentrations.
- Controls: Dilution water
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): None observed in final test solutions. - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Unicellular green algae
- Strain: ATCC22662
- Source (laboratory, culture collection): America Type Culture Collection
- Age of inoculum (at test initiation): Original strain at lab over 10 years old. This is periodically tested to ensure the algae is not comtaminated. Algae was subcultured up to 4 days ahead of use in test conditions.
- Method of cultivation: Under the same conditions as that during the test. No modified or unusual cells were observed.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 22 +/- 2°C (in culturing apparatus)
- pH:
- Beginning in test vessels: 7.8 to 7.9
End in test vessels: 8.1 to 10.4
The pH of the control vessels increased by more than 1.5 units over the course of the test which is outside the guideline requirements. Generally, the concentration of the bicarbonate ion decreases by carbon dioxide assimilation in algae. If there is little air exchange (supply of CO2) in a closed system the pH would increase. Since the algae grew exponentially in the control group, it was considered that the pH increase did not affect the integrity of the study. - Nominal and measured concentrations:
- Nominal: 0.1, 0.32, 1.0, 3.2 and 10 mg/L
Measured (time weighted mean): 0.0222, 0.0697, 0.211, 0.604 and 1.45 mg/L. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.42 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% confidence limits 0.409 to 0.432 mg/L
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.07 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): None
- Unusual cell shape: None
- Colour differences: In the controls and the first three test concentrations, the test cultures appeared to be green. The colour in the highest two test concentrations was unchanged.
- Flocculation: none
- Adherence to test vessels: Not observed
- Aggregation of algal cells: None
- Any stimulation of growth found in any treatment: None
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: None observed - Results with reference substance (positive control):
- - Results with reference substance valid? Results were within the range of historical laboratory data
- EC50: 0.793 mg/L - Reported statistics and error estimates:
- The NOEC was determined by analysis of variance (ANOVA), Williams's test subsequent to Bartlett's test for homogeniety of variances.
The EC50 value and 95% confidence limits were determined by least squares regression analysis of the logarithm of test concentration against the growth inhibition (%) relative to the control. - Validity criteria fulfilled:
- yes
- Remarks:
- The biomass of the controls increased exponentially (above 16 fold). The Coefficient of Variation parameters set in the OECD guideline were also not exceeded.
- Conclusions:
- An OECD guideline, GLP study assessing algal growth inhibition provided a 72 hour ErC50 value of 0.42 mg/L and a NOEC of 0.0697 mg/L.
Reference
Description of key information
A reliable OECD guideline and GLP study is available assessing algal growth inhibition of the substance. No data is available assessing the toxicity of the substance to marine algae.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 0.42 mg/L
- EC10 or NOEC for freshwater algae:
- 0.07 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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