2,2,4-trimethyl-1-oxa-4-aza-2-silacyclohexane

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: The exact LD50 was not determined; highest applied dose was 900 mg/kg bw (=1000 µl kg/bw).

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1969

Report date:

1969

Materials and methods

Principles of method if other than guideline:

The test substance was applied to the abdominal skin in a dose of 1000 µl/kg bw to 5 male rats for 4 hours. Additionally the test substance was applied in doses of 250, 500 or 1000 µl to the back skin to 5 male rats each for 7 hours. Afterwards the test substance was removed by water and soap and the animals were observed for 7 days.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

2,2,4-trimethyl-1-oxa-4-aza-2-silacyclohexane

EC Number:

233-487-5

EC Name:

2,2,4-trimethyl-1-oxa-4-aza-2-silacyclohexane

Cas Number:

10196-49-3

Molecular formula:

C6H15NOSi

IUPAC Name:

2,2,4-trimethyl-1,4,2-oxazasilinane

Specific details on test material used for the study:

Aktivator MO = 2,2,4-trimethyl-1-oxa-4-aza-2-silacyclohexane (0) was used as test substance.

Test animals

Species:

rat

Strain:

not specified

Sex:

male

Administration / exposure

Type of coverage:

open

Vehicle:

unchanged (no vehicle)

Duration of exposure:

Four or seven hours.

Doses:

1000 µl/kg bw for 4 hours (1000 µl = 900 mg)
250, 500 or 1000 µl/kg bw for 7 hours (= 225, 450 or 900 mg)

No. of animals per sex per dose:

5 male rats per dose

Control animals:

no

Details on study design:

The licking of the test item was prevented by a cardboard collar.

Results and discussion

Effect levels

Sex:

female

Dose descriptor:

LD50

Effect level:

> 900 mg/kg bw

Based on:

test mat.

Mortality:

With 500 µl/kg bw and 1000 µl kg/bw 1 of 5 aninimals died.

Clinical signs:

Decrease of the general condition.

Applicant's summary and conclusion

Interpretation of results:

study cannot be used for classification

Conclusions:

At a dose of 1000 µl/kg bw for 4 hours (1000 µl = 900 mg) no animals died.
At a dose of 250, 500 or 1000 µl/kg bw for 7 hours (= 225 mg) no animals died.
At a dose of 500 or 1000 µl/kg bw for 7 hours (450 or 900 mg) 1 out of 5 animals died.
The exact LD50 could no be determined. However in each case it is greater than 900 mg/kg bw (=1000 µl kg/bw).

Executive summary:

The test substance was applied to the abdominal skin in a dose of 1000 µl/kg bw to 5 male rats for 4 hours. Additionally the test substance was applied in doses of 250, 500 or 1000 µl to the back skin to 5 male rats each for 7 hours. Afterwards the test substance was removed by water and soap and the animals were observed for 7 days. At a dose of 500 or 1000 µl/kg bw for 7 hours (450 or 900 mg) 1 out of 5 animals died. Therefore the LD50 is greatr than 1000 µl/kg bw (900 mg/kg bw). However the exact LD50 was not determined in the study. According to CLP classification criteria (Regulation (EC) No 1272/2008) the study cannot be used for classification.