Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1995
Report date:
1995

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: Directive 92/69/EEC
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
4-tert-butyltoluene
EC Number:
202-675-9
EC Name:
4-tert-butyltoluene
Cas Number:
98-51-1
Molecular formula:
C11H16
IUPAC Name:
1-tert-butyl-4-methylbenzene
Details on test material:
- Name of test material (as cited in study report): p-tert-butyltoluene
- Analytical purity: 96.3 %
- Lot/batch No.: no batch No., Reaktor C 510

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 1g/l of the test substance was dissolved in synthetic test water and stirred for 18 hours. Subsequently the solution was filtered and the DOC was measured. DOC: 6.3 mg/l

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Age at study initiation (mean and range, SD): < 24 h

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Test temperature:
20 ± 1 °C
Nominal and measured concentrations:
Nominal test concentrations:
control, 0.7, 1.2, 2.1, 3.5 and 6.3 mg/l

Measured test concentrations after 0 h:
control, 1.2, 1.6, 2.3, 3.7 and 6.6 mg/l

Measured test concentrations after 48 h:
control, 0.44, 0.75, 1.0, 1.6 and 2.7 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel: glass vessels (10 ml)
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates):


OTHER TEST CONDITIONS
- Photoperiod: continous darkness

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
mobility after 0 ,24 and 48 h

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
2.4 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
3.2 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 4.2 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
mobility

Applicant's summary and conclusion