4-tert-butyltoluene

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Directive 92/69/EEC

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

4-tert-butyltoluene

EC Number:

202-675-9

EC Name:

4-tert-butyltoluene

Cas Number:

98-51-1

Molecular formula:

C11H16

IUPAC Name:

1-tert-butyl-4-methylbenzene

Details on test material:

- Name of test material (as cited in study report): p-tert-butyltoluene
- Analytical purity: 96.3 %
- Lot/batch No.: none, Reaktor C 510

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution was prepared by adding 1 g/l to deionised -water and stirred for 18 hours. The solution was filtered.

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Source (laboratory, culture collection): originally obtained from the Institut fuer Wasser-, Boden- und Lufthygiene, Berlin, Germany. Cultured at the testing facility

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Test temperature:

21.4 - 2.2 °C

pH:

at test start 8.8 - 8.9
at test end: 8.6 - 9.2

Nominal and measured concentrations:

Nominal test concentration:
control, 0.62, 1.1, 1.9, 3.1, 5.6 mg/l

Measured concentration after 72 hours:
control, 0.41, 0.86, 1.6, 3.1, 5.3 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: Erlenmeyer vessel
- Initial cells density: 1000
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 8


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to 92/69/EWG


OTHER TEST CONDITIONS
- Light intensity and quality: white light, ca. 8000 Lux


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: photometric, adsorption at a wave length of 685 nm

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

>= 5.6 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

other: biomass and growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 5.6 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

other: biomass and growth rate