Oligomerisation products of ethylene oxide with reaction products of rape oil and ethanolamine

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

from 10th February 2003 to 16th May 2003

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

Water samples were taken at 0, 24, 48, 72 and 96 h for quantitative analysis.

Details on test solutions:

Samples are extracted with dichloromethane, dryed with sodium sulphate and the sovent was evaporated. Then the analysis was done in as solution in acetone.

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

Fish were obtained from Brow Well Fisheries, Hebden, UK. Fish were maintained in a glass fibre tank.

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

Sub-lethal effects were observed at concentrations of 3.2 mg/L and above. These responses were fish swimming at the bottom of the test vessel, swimming at the surface, loss of equilibrium and presence of moribund fish.

Hardness:

133 mg/L as CaCO3

Test temperature:

14 °C

pH:

7.6 - 7.7

Dissolved oxygen:

81-91 air saturation value

Salinity:

441 µS/m

Nominal and measured concentrations:

Nominal Measured
1 0.466
1.8 0.766
3.2 1.64
5.6 5.2
10 8.68

Details on test conditions:

Concentrations for the definitive tests are chosen according to the results for the range-finding test. LC50-values are calculated from the results using various statistical methods.

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

0.77 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: The NOEC is based upon zero mortalities and the absence of any sub-lethal effects of exposure at this concentration

Duration:

96 h

Dose descriptor:

other: 95% CL

Effect conc.:

>= 1.6 - <= 5.2 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL. Concentrations resulting in 0% and 100% mortalities respectively

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

2.9 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Given the decline in measured test concentrations it was considered justifiable to base the results on the time-weighted mean measured concentrations of the test media to give a "worst case" analysis of the data.

Validity criteria fulfilled:

yes

Conclusions:

The acute toxicity of the test material to the freshwater fish rainbow trout (Oncorhynchus mykiss) has been investigated. Based on the time-weighted mean measured test concentrations the acute toxicity of the test material to rainbow trout gave a 96-Hour LC50 value of 2.9 mg/L with 95% confidence limits of 1.6-5.2 mg/L. The No Observed Effect Concentration was 0.77 mg/L.

Executive summary:

A study was performed to assess the acute toxicity of the test material to rainbow trout (Oncorhynchus mykiss). The method followed that described in the OECD guidelines for Testing of Chemicals (1992) No. 203, “Fish, Acute Toxicity Test” referenced as Method C.1 of Commission Directive 92/69/EEC.

Following a preliminary range-finding test, fish were exposed, in groups of twenty, to an aqueous dispersion of the test material over a range of concentrations of 1.0, 1.8, 3.2, 5.6 and 10mg/L for a period of 96 hours at a temperature of 13.9 to 14.5 °C under semi-static test conditions. The test was conducted in the dark. The number of mortalities and any sub-lethal effects of exposure in each test vessel and control vessel were determined 3 and 6 hours after the start of exposure and then daily throughout the test until termination after 96 hours.

 

Given the decline in measured test concentrations it was considered justifiable to base the results on the time-weighted mean measured test concentrations of the test media to give a “worst case” analysis of the data. The 96-Hour LC50 based on the time weighted mean measured test concentrations was 2.9 mg/L with 95% confidence limits of 1.6-5.2 mg/L. The No Observed Effect Concentration was 0.77 mg/L.

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

From 6 September 1991 to 25 October 1991

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

Concentrations for the definitive tests are chosen according to the results for the range-finding test. LC50-values are calculated from the results using various statistical methods.

Test organisms (species):

Leuciscus idus melanotus

Details on test organisms:

TEST ORGANISM
- Strain: Leuciscus idus melanotus
- Source: Fischzucht Eggers, W-2354 Hohenwestedt
- Length at study initiation (length definition, mean, range and SD): 5-8 cm

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

20

pH:

8.38

Dissolved oxygen:

7.88

Details on test conditions:

Preparation of stock solution with a concentration of 1g/L of test substance by dilution with drinking water.

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

10 mg/L

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

13 mg/L

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

16 mg/L

Validity criteria fulfilled:

yes

Conclusions:

LC50(96h)= 13 mg/L

Executive summary:

According to OECD Guideline 203 (Fish, Acute toxicity Test)

The acute toxicity of test material to the Leuciscus idus melanotus was determinate in a 96 -hour, static, aquatic effects test. A total of 10 fishes were exposed to each of six test substance concentrations. Concentrations were 2, 4, 10, 14 and 16 mg/L. The LC50(96h) was 13 mg/L.

Description of key information

According to OECD Guideline 203 (Fish, Acute toxicity Test).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

2.9 mg/L

Additional information

There are two studies available for this endpoint. The key study was chosen as the study was performed under GLP-conditions (reliability 1). This study was additionally resulting in a higher toxicity of the substance and represents the worst case for acute toxicity to fish. The key study gave an LC50(96h) of 4.2 mg/L (based on nominal test concentrations). The LC50 (96h) based on time-weighted mean measured test concentrations was 2.9 mg/L.