Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 279-116-0 | CAS number: 79234-33-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Endpoint summary
Administrative data
Description of key information
Skin Sensitization:
The skin sensitization potential of 4-(phenylazo)benzene-1,3-diamine acetate was estimated by SSS (2018) using OECD QSAR toolbox v3.3 with log kow as the primary descriptor. 4-(phenylazo)benzene-1,3-diamine acetate was predicted to be sensitizing to the skin of male Hartley guinea pigs.
Key value for chemical safety assessment
Skin sensitisation
Link to relevant study records
- Endpoint:
- skin sensitisation
- Remarks:
- in vivo
- Type of information:
- (Q)SAR
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- results derived from a valid (Q)SAR model and falling into its applicability domain, with limited documentation / justification
- Justification for type of information:
- data is from OECD QSAR toolbox v3.3 and the QMRF report has been attached
- Qualifier:
- according to guideline
- Guideline:
- other: Estimated data
- Principles of method if other than guideline:
- Prediction was done using OECD QSAR toolbox v3.3
- GLP compliance:
- not specified
- Type of study:
- other: Estimated data
- Specific details on test material used for the study:
- - Name of test material: 4-(phenylazo)benzene-1,3-diamine acetate
- IUPAC name: 4-(phenylazo)benzene-1,3-diamine acetate
- Molecular formula: C14H16N4O2
- Molecular weight: 272.306 g/mole
- Smiles Notation: c1(ccc(c(N)c1)\N=N\c1ccccc1)N.C(C)(=O)O
- InChl: 1S/C12H12N4.C2H4O2/c13-9-6-7-12(11(14)8-9)16-15-10-4-2-1-3-5-10;1-2(3)4/h1-8H,13-14H2;1H3,(H,3,4)/b16-15+;
- Substance type: Organic
- Physical state: Solid - Species:
- guinea pig
- Strain:
- Hartley
- Sex:
- male
- Details on test animals and environmental conditions:
- no data available
- Route:
- epicutaneous, open
- Vehicle:
- not specified
- Adequacy of induction:
- not specified
- No.:
- #1
- Route:
- intradermal
- Vehicle:
- not specified
- Adequacy of challenge:
- not specified
- No. of animals per dose:
- 10
- Details on study design:
- no data available
- Challenge controls:
- no data available
- Positive control substance(s):
- not specified
- Positive control results:
- no data available
- Other effects / acceptance of results:
- no data available
- Reading:
- 1st reading
- Group:
- test chemical
- Clinical observations:
- signs of dermal sensitization observed
- Remarks on result:
- positive indication of skin sensitisation
- Interpretation of results:
- other: Positive
- Conclusions:
- 4-(phenylazo)benzene-1,3-diamine acetate was predicted to be sensitizing to the skin of male Hartley guinea pigs
- Executive summary:
The skin sensitization potential of 4-(phenylazo)benzene-1,3-diamine acetate was estimated by SSS (2018) using OECD QSAR toolbox v3.3 with log kow as the primary descriptor. 4-(phenylazo)benzene-1,3-diamine acetate was predicted to be sensitizing to the skin of male Hartley guinea pigs.
Reference
The
prediction was based on dataset comprised from the following
descriptors: "Skin Sensitisation"
Estimation method: Takes highest mode value from the 6 nearest neighbours
Domain logical expression:Result: In Domain
((((((((((((("a"
or "b" or "c" or "d" )
and ("e"
and (
not "f")
)
)
and ("g"
and (
not "h")
)
)
and ("i"
and (
not "j")
)
)
and ("k"
and (
not "l")
)
)
and ("m"
and (
not "n")
)
)
and ("o"
and (
not "p")
)
)
and ("q"
and (
not "r")
)
)
and ("s"
and (
not "t")
)
)
and ("u"
and (
not "v")
)
)
and ("w"
and (
not "x")
)
)
and ("y"
and (
not "z")
)
)
and ("aa"
and "ab" )
)
Domain
logical expression index: "a"
Referential
boundary: The
target chemical should be classified as Anilines (Acute toxicity) AND
Not categorized by US-EPA New Chemical Categories
Domain
logical expression index: "b"
Referential
boundary: The
target chemical should be classified as SN1 AND SN1 >> Nitrenium Ion
formation AND SN1 >> Nitrenium Ion formation >> Aromatic azo AND SN1 >>
Nitrenium Ion formation >> Primary aromatic amine by DNA binding by OECD
Domain
logical expression index: "c"
Referential
boundary: The
target chemical should be classified as Strong binder, NH2 group by
Estrogen Receptor Binding
Domain
logical expression index: "d"
Referential
boundary: The
target chemical should be classified as Anilines (amino-meta) AND
Anilines (Unhindered) by Aquatic toxicity classification by ECOSAR
Domain
logical expression index: "e"
Referential
boundary: The
target chemical should be classified as Non binder, non cyclic structure
AND Strong binder, NH2 group by Estrogen Receptor Binding
Domain
logical expression index: "f"
Referential
boundary: The
target chemical should be classified as Very strong binder, OH group by
Estrogen Receptor Binding
Domain
logical expression index: "g"
Referential
boundary: The
target chemical should be classified as Non binder, non cyclic structure
AND Strong binder, NH2 group by Estrogen Receptor Binding
Domain
logical expression index: "h"
Referential
boundary: The
target chemical should be classified as Non binder, MW>500 by Estrogen
Receptor Binding
Domain
logical expression index: "i"
Referential
boundary: The
target chemical should be classified as Aliphatic acids and (Met)acrylic
acids AND Aromatic amines by Skin irritation/corrosion Inclusion rules
by BfR
Domain
logical expression index: "j"
Referential
boundary: The
target chemical should be classified as Inclusion rules not met by Skin
irritation/corrosion Inclusion rules by BfR
Domain
logical expression index: "k"
Referential
boundary: The
target chemical should be classified as Aliphatic acids and (Met)acrylic
acids AND Aromatic amines by Skin irritation/corrosion Inclusion rules
by BfR
Domain
logical expression index: "l"
Referential
boundary: The
target chemical should be classified as Ketones OR Phenols by Skin
irritation/corrosion Inclusion rules by BfR
Domain
logical expression index: "m"
Referential
boundary: The
target chemical should be classified as Non-Metals by Groups of elements
Domain
logical expression index: "n"
Referential
boundary: The
target chemical should be classified as Alkali Earth OR Halogens by
Groups of elements
Domain
logical expression index: "o"
Referential
boundary: The
target chemical should be classified as Acetoxy AND Aminoaniline, meta
AND Aniline AND Aryl AND Azo AND Carboxylic acid by Organic Functional
groups
Domain
logical expression index: "p"
Referential
boundary: The
target chemical should be classified as Aliphatic Amine, secondary OR
Alkene by Organic Functional groups
Domain
logical expression index: "q"
Referential
boundary: The
target chemical should be classified as Acetoxy AND Aminoaniline, meta
AND Aniline AND Aryl AND Azo AND Carboxylic acid by Organic Functional
groups
Domain
logical expression index: "r"
Referential
boundary: The
target chemical should be classified as Benzamide by Organic Functional
groups
Domain
logical expression index: "s"
Referential
boundary: The
target chemical should be classified as Acetoxy AND Aminoaniline, meta
AND Aniline AND Aryl AND Azo AND Carboxylic acid by Organic Functional
groups
Domain
logical expression index: "t"
Referential
boundary: The
target chemical should be classified as Sulfone by Organic Functional
groups
Domain
logical expression index: "u"
Referential
boundary: The
target chemical should be classified as Acetoxy AND Aminoaniline, meta
AND Aniline AND Aryl AND Azo AND Carboxylic acid by Organic Functional
groups
Domain
logical expression index: "v"
Referential
boundary: The
target chemical should be classified as Sulfonamide by Organic
Functional groups
Domain
logical expression index: "w"
Referential
boundary: The
target chemical should be classified as Acetoxy AND Aminoaniline, meta
AND Aniline AND Aryl AND Azo AND Carboxylic acid by Organic Functional
groups
Domain
logical expression index: "x"
Referential
boundary: The
target chemical should be classified as Pyridine by Organic Functional
groups
Domain
logical expression index: "y"
Referential
boundary: The
target chemical should be classified as Acetoxy AND Aminoaniline, meta
AND Aniline AND Aryl AND Azo AND Carboxylic acid by Organic Functional
groups
Domain
logical expression index: "z"
Referential
boundary: The
target chemical should be classified as Carboxylic acid ester by Organic
Functional groups
Domain
logical expression index: "aa"
Parametric
boundary:The
target chemical should have a value of log Kow which is >= -0.513
Domain
logical expression index: "ab"
Parametric
boundary:The
target chemical should have a value of log Kow which is <= 2.22
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed (sensitising)
- Additional information:
Skin Sensitization:
Various studies have been investigated for assessing the dermal sensitization potential of 4-(phenylazo)benzene-1,3-diamine to a greater or lesser extent. The studies are based on in vivo experiments in guinea pigs as well predicted data for target chemical and its structurally similar read across chemicals, 4-nitrobenzene-1,3-diamine[CAS: 5131-58-8] and 4-Methoxybenzylamine[CAS: 2393-23-9]. The predicted data has also been compared with the experimental data.
The skin sensitization potential of 4-(phenylazo)benzene-1,3-diamine acetate was estimated by SSS (2018) using OECD QSAR toolbox v3.3 with log kow as the primary descriptor. 4-(phenylazo)benzene-1,3-diamine acetate was predicted to be sensitizing to the skin of male Hartley guinea pigs.
This is supported by an experimental study summarized in GESTIS - Substance Database (Information system on hazardous substances of the Berufsgenossenschaften), 2011 for the structurally similar read across chemical, 4-nitrobenzene-1,3-diamine[CAS: 5131-58-8].
A test on the skin-sensitizing effects of the substance on guinea pigs yielded positive results and confirmed a moderate allergic potential. Therefore 4-nitrobenzene-1,3-diamine( 5131-58-8) was considered to be sensitizing in Guinea pig.
Guinea pig Maximization assay was performed (OTS0546455, NTRL report, 1992) to determine the degree of dermal sensitization caused by the structurally similar read across chemical, 4-Methoxybenzylamine[CAS: 2393-23-9]. 10 female Hartley guinea pigs were used for the study.
During the induction phase, the test chemical in Freund's Adjuvant was injected into the skin of guinea pigs, then 1% of the test material was applied onto the skin. Negative control was also used (name not specified). After few days, the induced guinea pigs were challenged with 1% test chemical and observed for signs of erythema.
5 animals showed slightly greater degree of erythema than control animals. Five of ten animals induced with the test material in Freund's Adjuvant responded to challenge with a slightly greater degree of erythema than seen in animals previously injected with Freund's Adjuvant only.
Based on these observations, 4-Methoxybenzylamine was considered to be sensitizing to guinea pig skin.
Based on the available data for the target as well as read across chemicals and applying the weight of evidence approach,4-(phenylazo)benzene-1,3-diamine was considered to sensitizing to skin.
Respiratory sensitisation
Endpoint conclusion
- Endpoint conclusion:
- no study available
Justification for classification or non-classification
Available data for 4-(phenylazo)benzene-1,3-diamine acetate indicates that it is likely to cause dermal sensitization to skin.
4-(phenylazo)benzene-1,3-diamine acetate can be considered to be sensitizer to skin.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
