Reaction products of diazotized 4-amino-6-[(4-aminophenyl)diazenyl]-3-[(4-aminophenyl)diazenyl]-5-hydroxynaphthalene-2,7-disulfonic acid, coupled with benzene-1,3-diamine, sodium salts

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Reaction products of diazotized 4-amino-6-[(4-aminophenyl)diazenyl]-3-[(4-aminophenyl)diazenyl]-5-hydroxynaphthalene-2,7-disulfonic acid, coupled with benzene-1,3-diamine, sodium salts

EC Number:

812-037-7

Cas Number:

1793011-72-9

Molecular formula:

Unknown for all components

IUPAC Name:

Reaction products of diazotized 4-amino-6-[(4-aminophenyl)diazenyl]-3-[(4-aminophenyl)diazenyl]-5-hydroxynaphthalene-2,7-disulfonic acid, coupled with benzene-1,3-diamine, sodium salts

Test material form:

solid

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 100 mg/L (nominal)
- Sampling method: One set was collected (fresh and old medium). Samples were taken from the middle layer of the relevant vessels.

Test solutions

Vehicle:

no

Details on test solutions:

As the test substance was soluble a 100 mg/L solution was prepared by adding the appropriate amount of substance to water in a 1 L volumetric flask. This was then inverted and stirred for 5 minutes.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Source: In house cultures
- Feeding during test: No

ACCLIMATION
- Acclimation period: 28 days or less
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: Chlorella vulgaris 6 mgC (organic carbon) /2 L/day
- Feeding frequency: As above
- Health during acclimation (any mortality observed): None observed

METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES: Juveniles less than 24 hours old were used for the test.

Study design

Test type:

semi-static

Water media type:

saltwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

Not specified

Test temperature:

20°C in test and control replicates

pH:

Within the range 7.7 and 8.2.

Dissolved oxygen:

Within the range 8.5 and 8.9 D.O mg/L

Salinity:

Not specified

Conductivity:

Not specified

Nominal and measured concentrations:

Nominal loading rate: 100 mg/L
Mean measured: 99.9 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 mL vessel
- Type: open
- Aeration: None
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Elendt M4 media as per OECD guideline

OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 hour light/ 8 hour dark
- Light intensity: 800 Lux or less

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Immobility (lack of movement within 15 seconds of gentle agitation) assessed at 24 and 48 hours.

RANGE-FINDING STUDY
- Test concentrations: 0 and 100 mg/L
- Results used to determine the conditions for the definitive study: Yes , no evidence of immobilisation observed in the range-finder.

Reference substance (positive control):

yes

Remarks:

Potassium dichromate test conducted every 6 months

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

No immobility was observed at a limit concentration of 100 mg/L. The mean test concentration at this concentration was 99.9 mg/L. The test solutions used on study were black with no precipitate evident although roughly 50% or more of the vessel surfaces were covered in an oily slick.

Results with reference substance (positive control):

- Results with reference substance valid: Yes (within historical laboratory range)

Reported statistics and error estimates:

Not applicable as a limit test

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

validity criteria on test fulfilled

Conclusions:

Under the conditions of the test no immobility was observed at the limit concentration of 100 mg/L to Daphnia magna and accordingly the 48 hour EC50 value was > 100 mg/L. The measured concentrations were near nominal.