Registration Dossier
Registration Dossier
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EC number: 252-563-9 | CAS number: 35441-13-5
Toxicity to aquatic plants other than algae
Administrative data
Link to relevant study record(s)
Description of key information
ErC50 > 100 mg/l based on nominal concentration (corresponding to 0.016 mg/l measured)
Key value for chemical safety assessment
Additional information
The substance was tested on Lemna Gibba according to the OECD guideline 221. A limit test was conducted at a single concentration at saturation (corresponding to 100 mg/L nominal concentration) using concurrent control ran. The exposure concentration of the saturated test solution was analytically determined to be 0.016 mg/L (calculated as the geometric mean of start and end concentrations measured at each renewal period).
The plants were exposed to the test substance for 7 days in a semi-static system (24-h water renewal periods).
In this 168-hour semi-static growth inhibition test on Lemna gibba the test item Fluorescent Brightener 313 had no toxic effect at aquatic saturation (measured value: 0.016 mg/L; equivalent to 100 mg/L nominal concentration).
Accordingly, the 168-h EC10, EC20, EC50 and the LOEC values based on growth rate and yield were determined to be > 0.016 mg/L (equivalent to 100 mg/L nominal concentration). The 168-h NOEC values were determined to be 0.016 mg/L (equivalent to 100 mg/L nominal concentration).
All validity criteria were met.
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