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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2016
Report date:
2016

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
N,N'-diphenyl-p-phenylenediamine
EC Number:
200-806-4
EC Name:
N,N'-diphenyl-p-phenylenediamine
Cas Number:
74-31-7
Molecular formula:
C18H16N2
IUPAC Name:
N,N'-diphenyl-p-phenylenediamine
Test material form:
solid

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
- Laboratory culture: Activated sludge from the municipal wastewater treatment plant of Mannheim, Germany was collected from the aeration tank of the plant
- Preparation of inoculum for exposure: The activated sludge suspension was sieved with a fine woven mesh (mesh size about 1 mm). This suspension was pre-aerated over night at room temperature. At the next day the sludge suspension was washed once with tap water and the suspension was adjusted to 7.5 g/L dry matter.
- Pretreatment: None
- Initial biomass concentration: 1.5 g/L dry mass

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
180 min

Test conditions

Details on test conditions:
TEST SYSTEM
- Test vessel: Erlenmeyer-flasks (nominal volume 250 mL)
- Type (delete if not applicable): closed
- Aeration: incubation was started by aeration of the test vessels with pressure air
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates):2
- No. of vessels per vehicle control (replicates):0

OTHER TEST CONDITIONS
- Adjustment of pH: no

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Justification for using less concentrations than requested by guideline:
- Test concentrations: 62.5, 125, 250, 500, 1000 mg/L
Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol

Results and discussion

Effect concentrationsopen allclose all
Duration:
180 min
Dose descriptor:
EC10
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
180 min
Dose descriptor:
other: EC20
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
180 min
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Results with reference substance (positive control):
The EC50 of 3,5-dichlorophenol is in the range of 5 - 30 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes