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Ecotoxicological information

Toxicity to microorganisms

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Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Remarks:
experimental data of read across substances
Justification for type of information:
Data for the target chemical is summarized based on the structurally similar read across chemicals
Reason / purpose for cross-reference:
read-across source
Reason / purpose for cross-reference:
read-across source
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Principles of method if other than guideline:
WoE report is based on two toxicity to microorganism studies as-
WoE 2. and WoE 3.
GLP compliance:
not specified
Analytical monitoring:
no
Remarks:
WoE 2: no and WoE 3: no data available
Details on sampling:
WoE 2: - Concentrations: Test chemical concentration used for the study were 1, 3.2, 10, 32 and 100 mg/l.
WoE 3: no data available
Vehicle:
not specified
Test organisms (species):
activated sludge, domestic
Details on inoculum:
WoE 2: - Source: Test organism activated sludge was obtained from laboratory sewage treatment apparatus (Husman).
WoE 3: no data available
Test type:
static
Water media type:
freshwater
Total exposure duration:
3 h
Test temperature:
WoE 2: 21.1°C
WoE 3: no data available
Reference substance (positive control):
yes
Remarks:
WoE 2: 3,5-dichlorophenol was used as a reference substance and WoE 3: no data available
Duration:
3 h
Dose descriptor:
EC0
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks on result:
other: WoE 2
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks on result:
other: WoE 2 and WoE 3
Details on results:
WoE 2: A significant inhibition of the respiration rate was not observed at any concentration.
WoE 3: no data available
Results with reference substance (positive control):
WoE 2: The IC50 value of the reference substance 3,5-dichlorophenol was determined to be within 10-25 mgO2/l.
WoE 3: no data available
Validity criteria fulfilled:
not specified
Conclusions:
On the basis of the experimental studies of the read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism activated sludge, the 3 hr EC50 value can be expected to be > 100 mg/l.
Executive summary:

Data available of the read across chemicals has been reviewed to determine the effect of the test chemical on toxicity to microorganisms. The studies are as mentioned below:

Toxicity to micro-organism study was carried out for 3 hours. The study was performed following the OECD Guideline 209 "Activated Sludge, Respiration Inhibition Test" at 21.1°C temperature. Test organism activated sludge (domestic) was obtained from laboratory sewage treatment apparatus (Husman). Test chemical concentration used for the study were 1, 3.2, 10, 32 and 100 mg/l. The test concentrations were not verified analytically. 3,5 -dichlorophenol was used as a reference substance. Oxygen-consumption was measured with an electrode system. The IC50 value of the reference substance 3,5 -dichlorophenol was determined to be within 10 -25 mgO2/l. No significant inhibition of the respiration rate was observed at any concentration. Thus, on the basis of effect on respiration rate of the test organism activated sludge, the EC0 and EC50 value was determined to be 100 and > 100 mg/l.

For the test chemical, toxicity to micro-organism study was carried out for 3 hours. The study was performed following the OECD Guideline 209 "Activated Sludge, Respiration Inhibition Test". Activated sludge (domestic) was used as a test organism. On the basis of effect on respiration rate of the test organism, the EC50 value was determined to be > 100 mg/l.

On the basis of the experimental studies of the read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism activated sludge, the 3 hr EC50 value can be expected to be > 100 mg/l.

Description of key information

On the basis of the experimental studies of the read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism activated sludge, the 3 hr EC50 value can be expected to be > 100 mg/l.

Key value for chemical safety assessment

Additional information

Data available of the read across chemicals has been reviewed to determine the effect of the test chemical on toxicity to microorganisms. The studies are as mentioned below:

 

Toxicity to micro-organism study was carried out for 3 hours. The study was performed following the OECD Guideline 209 "Activated Sludge, Respiration Inhibition Test" at 21.1°C temperature. Test organism activated sludge (domestic) was obtained from laboratory sewage treatment apparatus (Husman). Test chemical concentration used for the study were 1, 3.2, 10, 32 and 100 mg/l. The test concentrations were not verified analytically. 3,5 -dichlorophenol was used as a reference substance. Oxygen-consumption was measured with an electrode system. The IC50 value of the reference substance 3,5 -dichlorophenol was determined to be within 10 -25 mgO2/l. No significant inhibition of the respiration rate was observed at any concentration. Thus, on the basis of effect on respiration rate of the test organism activated sludge, the EC0 and EC50 value was determined to be 100 and > 100 mg/l.

 

For the test chemical, toxicity to micro-organism study was carried out for 3 hours. The study was performed following the OECD Guideline 209 "Activated Sludge, Respiration Inhibition Test". Activated sludge (domestic) was used as a test organism. On the basis of effect on respiration rate of the test organism, the EC50 value was determined to be > 100 mg/l.

 

On the basis of the experimental studies of the read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism activated sludge, the 3 hr EC50 value can be expected to be > 100 mg/l.