(carboxylatomethyl)dodecyldimethylammonium

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Deviations:

yes

Remarks:

Due to a batch of defective soda-lime, the CO2 trap sequence was changed from 2 soda-lime traps to 2 soda-lime traps followed by 2 500ml dreschels of 3M NaOH and 1 dreschel of Maxima H2O to act as a caustic vapour trap on 30/03/17 (day 1).

GLP compliance:

yes

Specific details on test material used for the study:

Identification: ColaTeric LAB-70UP
Lot/batch number: 53983K16
Homogeneity (supplied): Homogeneous
Homogeneity (observed): Homogeneous
Date of receipt: 12 December 2016
Expiry date: 18-11-2018
Purity/Composition of test substance: CAS Number: 683-10-3
Lauryl Betaine 32 - 34%
Sodium Chloride 6 - 8%
Water 58 – 60%
Chemex reference: ECO161202
Required storage conditions: Ambient (15°C ±10°C)
Actual storage conditions: Ambient (15°C ±10°C)
Stability in container after opening: Stable
Quoted stability in water: Stable
Quoted appearance: Clear liquid (TSDS), Yellow liquid (MSDS)
Observed appearance: Clear yellow liquid

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

Source: Cambridge Sewage Treatment Works, Cowley Road
Date of collection: 28/03/17

Duration of test (contact time):

28 d

Initial conc.:

15 mg/L

Based on:

DOC

Initial conc.:

20 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation:

CO2 evolution

Reference substance:

acetic acid, sodium salt

Key result

Parameter:

% degradation (CO2 evolution)

Value:

60

Sampling time:

9 d

Key result

Parameter:

% degradation (CO2 evolution)

Value:

85

Sampling time:

29 d

Time (days)	Reference material (Sodium acetate)	Test material (ColaTeric LAB-70UP)
0	0	0
2	27	3
5	45	25
7	56	47
9	60	60
14	67	71
19	70	76
23	71	78
28	73	78
29	76	79
29	84	85

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Description of key information

THis substance gave a positive result (>60% degradation relative to the ThCO2 value) with a maximum of 85% recorded on day 29. It may therefore, be concluded that there is a potential for biodegradation under environmental conditions.

The test protocol requires that a 10 day window is applied to the degradation results (60% degradation to be reached within 10 days of 10% of the theoretical carbon dioxide produced).

10% degradation was achieved between day 2 and day 5, and 60% was reached by day 9, ColaTeric LAB-70UP therefore achieved the 10-day window.

The mean result of duplicate testing for biodegradability by OECD 301B (CO2 evolution test) is 60% biodegraded at 9 days, and 85% biodegraded at 29 days.

The final conclusion of this study is therefore that lauryl betaine is readily biodegradable in an aerobic aqueous environment.

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable