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EC number: 250-654-8 | CAS number: 31482-56-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 24 October 2017 to 26 October 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- Report states C.3; it is assumed that this is a typographical error
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Guidelines for studies on the new chemical substance
- Version / remarks:
- Guidelines for studies on the new chemical substance as required by the Law Concerning the Evaluation of Chemical Substances and Regulation of their Manufacture, etc (Chemical Substance Control Law) 1973, amended 2009 under the reference of YAKUSHOKHATSU No. 1121002, SEIKYOKU No.2 and KANPOKIHATSU No. 021121002 and partially amended 2006 as the joint ordinance of The Japanese Ministry of Economy Trade and Industry (METI), Ministry of Health, Labour and Welfare (MHLW) and Ministry of the Environment (MOE).
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: JMAFF 2-7-2-1
- Version / remarks:
- JMAFF 2-7-2-1 The guidelines related to the study reports for the registration application of pesticide (Ref. No. 12-Nousan-8147 on 24 November 2000) & Ref. No.13-Seisan-3986 on 10 October 2001
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: because the test material was very poorly soluble in water, a test solution was prepared using a saturated solution method according to the Guidance Document on Aquatic Toxicity Testing of Difficult Substances and Mixtures, OECD No. 23. A saturated test material solution (nominal loading rate of 100 mg/L) was prepared by dispersing/dissolving the amount of test material into the test medium (ISO Medium) two days before the start of the experiment. This solution was shaken for approximately 24 hours at approximately 30°C (Day -2) and then was equilibrated for approximately 24 hours at approximately 20°C (Day -1). The non-dissolved test materials were removed by filtration through a fine (0.22 μm) filter to give the 100% saturated solution. As only limit test was carried out, further dilution of this saturated solution was not performed. Test solution was distributed into test vessels prior to introduction of Daphnia. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Source: Szent István University, Department of Aquaculture 2100 Gödöllő, Páter Károly u. 1. – Hungary
ACCLIMATION
There was no acclimatisation because the water used was similar to the culture water. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 247 mg/L (as CaCO3)
- Test temperature:
- 20.1 - 20.6°C
- pH:
- 7.06 - 7.54
- Dissolved oxygen:
- 7.8 - 8.7 mg/L
- Nominal and measured concentrations:
- 100% saturated solution (because a significant toxic response was not observed during the range-finding test)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beakers
- Material, size, headspace, fill volume: at least 5 mL test solution/animal
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water (ISO medium, according to OECD 202) was used as dilution water for both the range finding and definitive tests. The same composition of reconstituted water was used for the tests and for breeding the test animals.
- Intervals of water quality measurement: The water temperature, the oxygen concentrations and pH of the controls and the test solution were measured at the beginning and at the end of the renewal periods.
OTHER TEST CONDITIONS
- Photoperiod: 16 hour light/8 hour darkness
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): immobility or mortality of Daphnia was determined by visual observation 24 and 48 hours after the start of the test. Those animals not able to swim within 15 seconds after gentle agitation of the beaker were considered to be immobile. The number of immobilised animals and the percentage of immobility was determined at 24 and 48 hours.
VEHICLE CONTROL PERFORMED: no
RANGE-FINDING STUDY
- Test concentrations: 0, 0.1, 1, 10 and 100 % saturated solution
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Remarks:
- For the evaluation of the quality of the Daphnia clone and validation of the experimental conditions, Potassium dichromate is tested at least twice a year to demonstrate satisfactory test conditions.
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 other: % saturated solution
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 100 other: % saturated solution
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 other: % saturated solution
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 100 other: % saturated solution
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- No animals were found to be immobile after 24 and 48 hours in both the control and test vessels.
- Reported statistics and error estimates:
- No statistical analysis was performed because of the lack of toxic effects. The EC50, NOEC, LOEC and EC100 values were determined directly from the raw data.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of the study, the test material had no toxic effect at saturation; the EC50 result is higher than the solubility of the test material in the test medium.
- Executive summary:
Acute toxicity of the test material on Daphnia magna was assessed in an acute immobilisation test, over an exposure period of 48 hours in a static test system. The study was conducted under GLP conditions and in accordance with the standardised guidelines OECD 202, EU Method C.2, US EPA OCSPP 850.1010 and JMAFF 2-7-2-1.
As toxic effects were not observed during the preliminary range-finding test, only one test concentration of 100% saturated solution (from a loading rate of 100 mg/L) and one control group was tested. The test was performed without analytical measurements because of the very poor water solubility of the test material. The test included twenty animals divided into four groups of five animals were used for the test concentration and the controls. No statistical analysis was performed because of the lack of toxic effects. The EC50, NOEC, LOEC and EC100 values were determined directly from the raw data. All validity criteria were met. The 48 h EC50, EC100, NOEC and LOEC are all 100% saturated solution.
Under the conditions of the study, the test material had no toxic effect at saturation; the EC50 result is higher than the solubility of the test material in the test medium.
Reference
Description of key information
Under the conditions of the study, the test material had no toxic effect at saturation; the EC50 result is higher than the solubility of the test material in the test medium.
Key value for chemical safety assessment
Additional information
Acute toxicity of the test material on Daphnia magna was assessed in an acute immobilisation test, over an exposure period of 48 hours in a static test system. The study was conducted under GLP conditions and in accordance with the standardised guidelines OECD 202, EU Method C.2, US EPA OCSPP 850.1010 and JMAFF 2-7-2-1.
As toxic effects were not observed during the preliminary range-finding test, only one test concentration of 100% saturated solution (from a loading rate of 100 mg/L) and one control group was tested. The test was performed without analytical measurements because of the very poor water solubility of the test material. The test included twenty animals divided into four groups of five animals were used for the test concentration and the controls. No statistical analysis was performed because of the lack of toxic effects. The EC50, NOEC, LOEC and EC100 values were determined directly from the raw data. All validity criteria were met. The 48 h EC50, EC100, NOEC and LOEC are all 100% saturated solution.
Under the conditions of the study, the test material had no toxic effect at saturation; the EC50 result is higher than the solubility of the test material in the test medium.
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