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EC number: 220-621-2 | CAS number: 2835-99-6
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
The 48 hour EC50 of 4-Amino-m-cresol to Daphnia magna was 0.20 mg/L (95% CL: 0.19 – 0.21 mg/L) based on time-weighted mean measured concentration.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.2 mg/L
Additional information
The 48 hour EC50 value of 4-amino-m-cresol to Daphnia magna was determined under semi-static conditions following procedures as described in the OECD guideline 202 acute toxicity for daphnia under GLP conditions.
4 replicates per test concentration with 5 daphnia per replicate were performed. The nominal tested concentrations were 0 (control), 0.10, 0.18, 0.32, 0.56 and 1.0 mg/L. These concentrations were analysed by LC-MS/MS on new and old media. Analysis of the freshly prepared test media at 0 and 24 hours showed measured concentrations to range from 102% to 112% of nominal. Analysis of the old media at 24 and 48 hours showed measured concentrations to range from 2% to 10% of nominal. Given the decline in measured concentration over each 24-hour exposure period, the results are expressed on time-weighted mean measured test concentrations. These were calculated to be 0.040, 0.060, 0.093, 0.15 and 0.26 mg/L and the percentage immobility (mortality) after 48h was 0, 20, 100, 100 and 100%, respectively.
The validity criteria for control immobility (≤10%) and dissolved oxygen (≥3 mg/L) at the end of the test were both satisfied.
The 48 hour EC50 of 4 -Amino--cresol to Daphnia magna was 0.20 mg/L (95% CL: 0.19 – 0.21 mg/L) based on time-weighted mean measured concentration.
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