3,7,11-Trimethyldodec-1-en-3-ol

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3,7,11-trimethyldodec-1-en-3-ol

EC Number:

609-221-2

Cas Number:

3625-46-5

Molecular formula:

C15H30O

IUPAC Name:

3,7,11-trimethyldodec-1-en-3-ol

Details on test material:

- Name of test material (as cited in study report): Tetrahydronerolidol (THNER)
- Lot/batch No.: Anlage 067, 13.06.01
- Analytical purity: 96%
water solubility: 15 mg/L

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

no stock solution was prepared

Test organisms

Test organisms (species):

activated sludge, domestic

Details on inoculum:

Preparation of the inoculum :
The inoculum was washed, brought to a concentration of 5 g/I dry substance and aerated during the night 50 ml were added to a total volume of 250 ml to obtain a concentration of 1 g/I dry substance in the test.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

180 min

Test conditions

Test temperature:

20 +/- 2 °C

pH:

7.6 after correction

Dissolved oxygen:

> 2.5 mg/L (during aeration)

Nominal and measured concentrations:

Nominal: 1000 mg/L + control (0 mg/L)

Details on test conditions:

TEST SYSTEM
- Test vessel: Erlenmeyer-vessel (nominal volume 250 ml)
- Fill volume: 250 ml
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: 8 ml/vessel 100-fold concentrated OECD medium



OTHER TEST CONDITIONS
- Adjustment of pH: yes

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

180 min

Dose descriptor:

other: EC20

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 2

Duration:

180 min

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Reported statistics and error estimates:

not applicable as the EC50 was above teh highest test concentration (i.e. above 1000 mg/L)