2-[[6-[(5-chloro-2,6-difluoro-4-pyrimidinyl)amino]-1-hydroxy-3-sulpho-2-naphthyl]azo]naphthalene-1,5-disulphonic acid, sodium salt

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: inherent biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1995

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)

Principles of method if other than guideline:

None

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Trisodium 2-[[6-[(4-amino-6-chloro-1,3,5-triazin-2-yl)methylamino]-1-hydroxy-3-sulphonato-2-naphthyl]azo]naphthalene-1,5-disulphonate

EC Number:

274-700-1

EC Name:

Trisodium 2-[[6-[(4-amino-6-chloro-1,3,5-triazin-2-yl)methylamino]-1-hydroxy-3-sulphonato-2-naphthyl]azo]naphthalene-1,5-disulphonate

Cas Number:

70616-89-6

Molecular formula:

C24H18ClN7O10S3.3Na

IUPAC Name:

trisodium 2-({6-[(4-amino-6-chloro-1,3,5-triazin-2-yl)(methyl)amino]-1-hydroxy-3-sulfonato-2-naphthyl}diazenyl)naphthalene-1,5-disulfonate

Test material form:

solid: particulate/powder

Specific details on test material used for the study:

None

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

Activated sludge of a communal sewage treatment plant Ara Reinach collected on 01.11.94

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

150 mg/L

Based on:

DOC

Details on study design:

Experimental conditionsWater / Nutrient: according to the specifications of the OECD guidelineTemperature: 20 - 25 °C (room temperature)Test duration: 28 daysLighting: indirect daylightEstimation Technique: TOC/DOC Analyzer 500

Reference substance

Reference substance:

diethylene glycol

Results and discussion

Preliminary study:

None

Test performance:

None

% Degradation

Key result

Parameter:

% degradation (DOC removal)

Value:

4

Sampling time:

28 d

Remarks on result:

other: Standard deviation not available

Details on results:

None

BOD5 / COD results

Results with reference substance:

The biodegradation of the reference substance after 8 days was: 98 %

Any other information on results incl. tables

None

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

Biodegradation of the test substance after 28 days was 4 %.

Executive summary:

A key study was performed to determine the percent biodegradation of FAT 40170. An inherent biodegradability of FAT 40170 was determined in a modified Zahn-Wellens - Test according to the OECD Guideline for Testing of Chemicals, No. 302B, 1992. The test substance was tested in a concentration of 150 mg/l. The reference substance (Diethylene glycol) was tested in a concentration of 150 mg/l. Based on the results of the study, biodegradation of the test substance after 28 days was 4 %.