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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2015
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2015
Report date:
2015

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
Principles of method if other than guideline:
not applicable
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
2-butoxyethyl benzoate
EC Number:
226-685-8
EC Name:
2-butoxyethyl benzoate
Cas Number:
5451-76-3
Molecular formula:
C13H18O3
IUPAC Name:
2-butoxyethyl benzoate
Test material form:
other: clear liquid
Details on test material:
- Name of test material (as cited in study report): 2-butoxyethyl benzoate (Butyl Cellosolve™ Benzoate)
- Physical state: clear liquid
- Analytical purity: 99.2%
- Lot/batch No.: 20130443-19
- Expiration date of the lot/batch: 02 April 2016
- Storage condition of test material: Ambient

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
A nominal concentration of 100 mg/L of test substance was prepared, and test solutions were made from dilutions of this stock.

Test organisms

Test organisms (species):
other: Gobiocypris rarus
Details on test organisms:
TEST ORGANISM
- Common name: Chinese rare minnow
- Strain: rarus
- Source: in house bred
- Water: the test water, tap water dechlorinated by activated carbon and sterilized by UV lamp was used
- Temperature: 21-25 °C and constant within a range of 2 °C
- pH: 6.0-8.5
- Oxygen concentration: > 80% ASV
- Photoperiod: 12 hours light/dark cycle
- Feeding: fishes were fed with Brine Shrimp Aquarium Flake Food, O.S.I® daily.

ACCLIMATION
- Acclimation period: more than 14 days
- Health during acclimation (any mortality observed): none noted

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Remarks on exposure duration:
The measured concentrations of the test substance still declined over each [ renewal period and thus concentrations varied more than 20%; therefore, the results were expressed using the time-weight mean (TWM) measured concentrations.

Test conditions

Test temperature:
22.1 - 23.4 C
Dissolved oxygen:
78.7 - 93.4 %
Nominal and measured concentrations:
0, 0.38, 0.65, 1.10, 1.90, and 3.0 mg/L nominal; 0.247, 0.426, 0.847, 1.14, amd 2.47 mg/L time-weighted measured concentrations
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Key result
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
1.09 mg/L
Nominal / measured:
meas. (TWA)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
The
96-h LC50 value for the test substance to Gobiocypris rarus was 1.09 mg/L and its 95% confidence limits
ranged from 0.766 to 1.48 mg/L. The maximum TWM mean measured concentration causing no
mortality and the minimum TWM mean measured concentration causing 100 percent mortality within the
period of the test were 0.426 mg/L and 2.47 mg/L, respectively.
Reported statistics and error estimates:
Standard statistical methods were employed

Any other information on results incl. tables

Sublethal observations / clinical signs:

None

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The
96-h LC50 value for the test substance to Gobiocypris rarus was 1.09 mg/L and its 95% confidence limits
ranged from 0.766 to 1.48 mg/L. The maximum TWM mean measured concentration causing no
mortality and the minimum TWM mean measured concentration causing 100 percent mortality within the
period of the test were 0.426 mg/L and 2.47 mg/L, respectively.
Executive summary:

The 96-h LC50 value for the test substance to Gobiocypris rarus was 1.09 mg/L and its 95% confidence limits

ranged from 0.766 to 1.48 mg/L. The maximum TWM mean measured concentration causing no

mortality and the minimum TWM mean measured concentration causing 100 percent mortality within the

period of the test were 0.426 mg/L and 2.47 mg/L, respectively.