Registration Dossier
Registration Dossier
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EC number: 226-685-8 | CAS number: 5451-76-3
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- Principles of method if other than guideline:
- not applicable
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2-butoxyethyl benzoate
- EC Number:
- 226-685-8
- EC Name:
- 2-butoxyethyl benzoate
- Cas Number:
- 5451-76-3
- Molecular formula:
- C13H18O3
- IUPAC Name:
- 2-butoxyethyl benzoate
- Test material form:
- other: clear liquid
- Details on test material:
- - Name of test material (as cited in study report): 2-butoxyethyl benzoate (Butyl Cellosolve™ Benzoate)
- Physical state: clear liquid
- Analytical purity: 99.2%
- Lot/batch No.: 20130443-19
- Expiration date of the lot/batch: 02 April 2016
- Storage condition of test material: Ambient
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
- Details on test solutions:
- A nominal concentration of 100 mg/L of test substance was prepared, and test solutions were made from dilutions of this stock.
Test organisms
- Test organisms (species):
- other: Gobiocypris rarus
- Details on test organisms:
- TEST ORGANISM
- Common name: Chinese rare minnow
- Strain: rarus
- Source: in house bred
- Water: the test water, tap water dechlorinated by activated carbon and sterilized by UV lamp was used
- Temperature: 21-25 °C and constant within a range of 2 °C
- pH: 6.0-8.5
- Oxygen concentration: > 80% ASV
- Photoperiod: 12 hours light/dark cycle
- Feeding: fishes were fed with Brine Shrimp Aquarium Flake Food, O.S.I® daily.
ACCLIMATION
- Acclimation period: more than 14 days
- Health during acclimation (any mortality observed): none noted
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Remarks on exposure duration:
- The measured concentrations of the test substance still declined over each [ renewal period and thus concentrations varied more than 20%; therefore, the results were expressed using the time-weight mean (TWM) measured concentrations.
Test conditions
- Test temperature:
- 22.1 - 23.4 C
- Dissolved oxygen:
- 78.7 - 93.4 %
- Nominal and measured concentrations:
- 0, 0.38, 0.65, 1.10, 1.90, and 3.0 mg/L nominal; 0.247, 0.426, 0.847, 1.14, amd 2.47 mg/L time-weighted measured concentrations
- Reference substance (positive control):
- no
Results and discussion
Effect concentrations
- Key result
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1.09 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- The
96-h LC50 value for the test substance to Gobiocypris rarus was 1.09 mg/L and its 95% confidence limits
ranged from 0.766 to 1.48 mg/L. The maximum TWM mean measured concentration causing no
mortality and the minimum TWM mean measured concentration causing 100 percent mortality within the
period of the test were 0.426 mg/L and 2.47 mg/L, respectively. - Reported statistics and error estimates:
- Standard statistical methods were employed
Any other information on results incl. tables
- Sublethal observations / clinical signs:
None
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The
96-h LC50 value for the test substance to Gobiocypris rarus was 1.09 mg/L and its 95% confidence limits
ranged from 0.766 to 1.48 mg/L. The maximum TWM mean measured concentration causing no
mortality and the minimum TWM mean measured concentration causing 100 percent mortality within the
period of the test were 0.426 mg/L and 2.47 mg/L, respectively. - Executive summary:
The 96-h LC50 value for the test substance to Gobiocypris rarus was 1.09 mg/L and its 95% confidence limits
ranged from 0.766 to 1.48 mg/L. The maximum TWM mean measured concentration causing no
mortality and the minimum TWM mean measured concentration causing 100 percent mortality within the
period of the test were 0.426 mg/L and 2.47 mg/L, respectively.
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