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EC number: 947-329-4 | CAS number: -
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 14 May - 12 Jun 2003
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: activated sludge from municipal wastewater treatment plant Breisgauer Bucht, Freiburg Region, Germany, capacity: 600000 inhabitant equivalents, sampled on 13th May 2003
- Pretreatment: Activated sludge was washed twice by settling the sludge, decanting the supernatant and resuspending the sludge in aerated tap water.
- Concentration of sludge: 4.5 g/L dry solids - Duration of test (contact time):
- 28 d
- Initial conc.:
- 8.12 mg/L
- Based on:
- other: organic carbon
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Remarks:
- via IC measurement
- Details on study design:
- TEST CONDITIONS
- Composition of medium: To a mineral medium, according to OECD 301B, 10 mL activated sludge (corresponding to 30 mg dry solids/L) was added. It was stirred overnight with a magnetic stirrer in a sealed CO2 free system (aeration with 2.7 - 5.5 bubbles CO2-free air/sec). Afterwards the test substance (8.12 mg organic carbon/L) was added. The stirring was continued over the whole test period.
- Test temperature: 22.0 - 24.0 °C
- Aeration of dilution water: with CO2 free air, aeration with 2.7 - 5.5 bubbles CO2-free air/sec
TEST SYSTEM
- Culturing apparatus: CO2-free air production system (reactor connected to two 1000 mL gas wash bottles filled with dry soda lime, followed by one bottle filled with 0.1 M sodium hydroxide, followed by one gas wash bottle filled with demineralised water, followed by an empty gas wash bottle to catch any drops of condensation water.)
- Number of culture flasks/concentration: 3 containing test item, 2 containing inoculum blank, 2 containing reference substance, 1 containing toxic reference
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Toxicity control: yes - Reference substance:
- benzoic acid, sodium salt
- Remarks:
- 51.3 - 52.4 mg (corresponding to 19.9 - 20.4 mg TOC/L)
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 95
- Sampling time:
- 14 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 100.5
- Sampling time:
- 28 d
- Details on results:
- The test substance was biodegraded by > 60%, within the 10-day time window. Thus, it can be considered as readily biodegradable.
- Results with reference substance:
- The reference substance degraded > 60% within the 10-day time window. Thus, the inoculum and test system can be considered valid.
- Interpretation of results:
- readily biodegradable
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 08 Aug - 06 Sep 2003
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: activated sludge from municipal wastewater treatment plant Breisgauer Bucht, Freiburg Region, Germany, capacity: 600,000 inhabitant equivalents, sampled on 30 Jun 2003
- Pretreatment: Activated sludge was washed twice by settling the sludge, decanting the supernatant and re-suspending the sludge in aerated tap water.
- Concentration of sludge: 3.9 g/L dry solids - Duration of test (contact time):
- 28 d
- Initial conc.:
- 20 mg/L
- Based on:
- other: organic carbon
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Remarks:
- via IC measurement
- Details on study design:
- TEST CONDITIONS
- Composition of medium: To a mineral medium according to OECD 301B, 30 mg/L activated sludge (corresponding to 20 mg organic carbon/L) was added. It was stirred overnight with a magnetic stirrer in a sealed CO2 free system (aeration with 2.7 - 5.5 bubbles CO2-free air/sec). Afterwards the test substance (15 g/L) was added. The stirring was continued over the whole test period.
- Test temperature: 21.3 - 23.0 °C
- Aeration of dilution water: with CO2 free air, aeration with 2.7 - 5.5 bubbles CO2-free air/sec
TEST SYSTEM
- Culturing apparatus: CO2-free air production system (reactor connected to two 1000 mL gas wash bottles filled with dry soda lime, followed by one bottle filled with 0.1 M sodium hydroxide, followed by one gas wash bottle filled with demineralised water, followed by an empty gas wash bottle to catch any drops of condensation water.)
- Number of culture flasks/concentration: 3 containing test item, 2 containing inoculum blank, 3 containing reference substance, 1 containing toxic reference
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Toxicity control: yes - Reference substance:
- benzoic acid, sodium salt
- Remarks:
- 20 mg organic carbon/L
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 89.3
- Sampling time:
- 28 d
- Details on results:
- The test substance was biodegraded by >60%, within the 10-day time window. Thus, it can be considered as readily biodegradable.
- Results with reference substance:
- The reference substance degraded > 60% within the 10-day time window. Thus, the inoculum and test system can be considered valid.
- Interpretation of results:
- readily biodegradable
Referenceopen allclose all
Table 1: Biodegradation of test substance, reference substance and toxic control
| Days after test start | Biodegradation [%] | ||
| test substance (mean of 3) | Reference substance (mean of 2) | Toxic reference | |
| 0 | 0 | 0 | 0 |
| 4 | 69.4 | 69.8 | 74.2 |
| 7 | 87.6 | 76 | 83.3 |
| 10 | 84.7 | 77.8 | 89.1 |
| 14 | 95 | 83.4 | 97.4 |
| 21 | 91.1 | 86.7 | 104.2 |
| 28 | 100.5 | 87.9 | 111.2 |
| 28 (after acidification) | 90.5 | 86.9 | 110.2 |
Table 1: Biodegradation of test substance, reference substance and toxic control
| Days after test start | Biodegradation [%] | ||
| Test substance (mean of 3) | Reference substance (mean of 3) | Toxic reference | |
| 0 | 0 | 0 | 0 |
| 4 | 41.4 | 73.1 | 43 |
| 7 | 62.7 | 80.6 | 52.3 |
| 10 | 79.6 | 86.1 | 73.9 |
| 14 | 82.5 | 82.5 | 75.9 |
| 20 | 85.4 | 96.1 | 84.8 |
| 28 | 88.7 | 101.3 | 82.6 |
| 28 (after acidification) | 89.3 | 101.7 | 87.8 |
Description of key information
Readily biodegradable according to OECD criteria: 100% after 28 days based on CO2 evolution (OECD 301 B).
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
Since no reliable biodegradability tests are available for Sulfuric acids, C9-11-iso-C10 rich, alkyl esters, sodium salt, in accordance to Regulation (EC) No. 1907/2006 Annex XI, 1.5 a read-across to other structurally related substances was conducted.
One GLP study assessing biodegradation of the C12-isoAS Na (CAS 94200-74-5), performed according to OECD guideline 301 B is available (Sasol, 2003). Activated, non-adapted domestic sludge was used as inoculum. The substance was tested in a concentration of 8.12 mg/L (as organic carbon) and CO2 evolution was measured. In result, 100% of biodegradation was observed after 28-day test period, passing 10-day window. It can be concluded that the substance is readily biodegradable.
A supporting GLP test was performed according to OECD guideline 301 B (Sasol, 2003) with CAS 126-92-1 (C8-isoAS Na). Activated, domestic, non-adapted sludge was used as inoculum and the initial concentration of the test substance was 20 mg/L expressed as organic carbon. CO2 evolution was measured for 28 days. Biodegradation reached 89.3% after 28 days and 79.6% after 10 days passing the 10-day window. It can be concluded that C8-isoAS Na (CAS 126-92-1) is readily biodegradable according to OECD criteria.
Due to structural and property similarities with the tested substances it can be concluded that the above results apply also to Sulfuric acids, C9-11-iso-C10 rich, alkyl esters, sodium salt. The substance can be considered as readily biodegradable according to OECD criteria.
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