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Short-term toxicity to aquatic invertebrates

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Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
21 Feb - 23 Feb 1995
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
yes
Analytical monitoring:
yes
Vehicle:
no
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Strain: Clone 5
- Source: in-house culture
- Age at study initiation: < 24 hours
- Method of breeding: parthenogenesis in Elendt M4 medium
- Feeding during test: no


ACCLIMATION
- Acclimation period: continuous
- Type and amount of food: Scenedesmus subspicatus
- Feeding frequency: daily

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
not relevant
Hardness:
no data
Test temperature:
20 ± 1 °C
pH:
8.0 - 8.1 (measured at test end)
Dissolved oxygen:
7.5 - 8.5 mg/L (measured at test end)
Nominal and measured concentrations:
nominal: 0, 90, 150, 280, 500 and 900 mg/L
measured geometric mean: 0, 45, 96, 176, 314 and 697 mg/L
Concentrations of the anionic active ingredient are given.
Details on test conditions:
TEST SYSTEM
- Test vessel: 10 mL glasses
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per reference control (replicates): 4
- Biomass loading rate: 2 mL per animal

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic freshwater
- Ca/Mg ratio: 4:1

OTHER TEST CONDITIONS
- Photoperiod: continuous dark

EFFECT PARAMETERS MEASURED: Immobilisation after 24 and 48 h.
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
176 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
act. ingr.
Remarks:
anionic active ingredient
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
483 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
act. ingr.
Remarks:
anionic active ingredient
Basis for effect:
mobility
Remarks on result:
other: 332-704 (95% C.L.)
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 697 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
act. ingr.
Remarks:
anionic active ingredient
Basis for effect:
mobility
Details on results:
- Behavioural abnormalities: Before complete immobilization, daphnids were observed to swim at the surface of the test vessels.
Results with reference substance (positive control):
- Results with reference substance valid? yes
- Mortality: 35% immobilisation at 1 mg/L potassium dichromate, 100% immobilisation at 2 mg/L potassium dichromate
Reported statistics and error estimates:
- EC50 calculation was conducted according to Probit analysis adapted from Cavalli-Sforza (1972)

Table 1: Immobilization of Daphnia magna after 24 and 48 h. The concentrations are provided as anionic active ingredient.

 

Measured concentration geometric mean [mg/L]

% Immobile

24 h

48 h

control

0

0

45

5

5

96

0

0

176

10

5

314

0

20

697

30

75

 

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Principles of method if other than guideline:
Method: other: AFNOR T.90301 (1974), no further information
GLP compliance:
no
Analytical monitoring:
no
Test organisms (species):
Daphnia magna
Details on test organisms:
Daphnia magna Straus
Water media type:
freshwater
Total exposure duration:
24 h
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
800 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods with acceptable restrictions
Principles of method if other than guideline:
Method: other: Norme Francaise Homologue, 1983, NF T90-31, no further information
GLP compliance:
no
Analytical monitoring:
no
Details on test solutions:
Distilled water solution of the surfactant was used.
Test organisms (species):
Daphnia magna
Water media type:
freshwater
Total exposure duration:
24 h
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
470 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Remarks:
Summary of available data used for the endpoint assessment of the target substance
Adequacy of study:
weight of evidence
Justification for type of information:
Refer to the Category Approach Justification document provided in IUCLID6 Section 13.
Reason / purpose for cross-reference:
read-across source
Reason / purpose for cross-reference:
read-across source
Reason / purpose for cross-reference:
read-across source
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
483
Nominal / measured:
meas. (geom. mean)
Conc. based on:
act. ingr.
Remarks:
anoinic active ingredient
Basis for effect:
mobility
Remarks on result:
other: Source, WoE, 126-92-1, 1995
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
800 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: Source, WoE, 142-87-0, 1978
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
470 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: Source, WoE, 142-87-0, 1991

Description of key information

48h EC50 (Daphnia magna, immobilization) > 100 mg/L

Key value for chemical safety assessment

Additional information

Since no reliable acute toxicity to aquatic invertebrates test is available for Sulfuric acids, C9-11-iso-C10 rich, alkyl esters, sodium salt, in accordance to Regulation (EC) No. 1907/2006 Annex XI, 1.5 a read-across to other structurally related substances was conducted. As the toxicity of alkyl sulfates is dependent on the carbon chain length, it is expected that the toxic effects are comparable for the similar homologues.

One GLP study investigating short-term toxic effects of C8-isoAS Na (CAS 126-91-2) to Daphnia magna is available (Elementis, 1995). The test was performed according to EU Method C.2 under static conditions. Inhibition of mobility of daphnids caused by the test substance was determined after 24 and 48 of static exposure to five test concentrations up to 697 mg a.i./L (measured concentration equal to 900 mg a.i./L of nominal concentration). The results show that the 48-hour EC50 value was 483 mg a.i./L (measured concentration).

Moreover two 24-hour tests with Daphnia magna are available for C10AS Na (CAS 142-87-0). The first non-GLP study followed a method described by Norme Francaise Homologue, 1983, NF T90-31 (Sanchez Leal 1991). The 24-hour EC50 value was 470 mg/L. Analytical check of the test concentrations was not provided. The other non-GLP study was performed following an AFNOR T.90301 (1974) method (Lundahl and Cabridenc 1978). The obtained results showed that the 24-hour EC50 value was 800 mg/L (nominal concentrations). Analytical measurements are not available.

Due to structural and property similarities with the tested similar substances, it can be concluded that the above results apply also to Sulfuric acids, C9-11-iso-C10 rich, alkyl esters, sodium salt. The 48-hour EC50 value for aquatic invertebrates is above 100 mg/L.