sulfuric acids, C9-11-iso-C10 rich, alkyl esters, sodium salt

Long-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

- Concentrations: control and all treatments
- Sampling method: The actual concentrations in the freshly prepared test solutions (0, 9 and 16 d) and aged test solutions (2, 12 and 19 d) were determined once a week.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The necessary amount of the substance was weighed into a weighing scoop, transferred to a volumetric flask and filled up to the bench mark with test medium. The lower test item solutions were made with test medium by dilution of the stock solutions. The test solution volume was 50 mL per test vessel. All solutions were prepared freshly on each day of application and renewal of test solutions.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Strain/clone: Clone V
- Source: originally obtained in a healthy condition from the Federal Environmental Agency in Berlin/Germany
- Age: freshly hatched daphnia of an age between 6 - 24 hours were used for the test
- Feeding during test: yes
- Food type: green algae (Desmodesmus subspicatus)
- Amount: 0.1 mg C/animal/day from day 1 to 7; 0.15 mg C/animal/day from day 8 to 14; 0.2 mg C/animal/day from day 15 to 21
- Frequency: at least three times a week

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Hardness:

11 - 15 °dH

Test temperature:

18.5 – 20.5 °C

pH:

7.15 – 9.08

Dissolved oxygen:

≥ 93 % of air saturation (approx. 9.3 mg O2/L)

Nominal and measured concentrations:

Nominal concentration: 0, 0.320, 1.00, 3.20, 10.0 and 32.0 mg/L
Measured concentration (TWA): 0, 0.079, 0.203, 0.497, 1.40 and 6.86 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: glass beakers
- Size, fill volume: 100 mL, 50 mL
- Aeration: none
- Renewal rate of test solution: 3 times a week
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10

TEST MEDIUM / WATER PARAMETERS
- Elendt M4 medium
- Intervals of water quality measurement: Measurements of temperature, pH-value and dissolved oxygen concentration were performed in the freshly prepared and in the aged test solutions of each renewal period (one vessel of each treatment group). The total hardness was determined in the test medium at each renewal.

OTHER TEST CONDITIONS
- Photoperiod: 16 hours photoperiod daily
- Light intensity: ~1500 lux

EFFECT PARAMETERS MEASURED: Mortality of adult animals, number of offspring and number of broods were recorded daily.

Reference substance (positive control):

no

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

1.4 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

reproduction

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

6.86 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

reproduction

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

32 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Details on results:

The 21d-EC50 (reproduction) is determined to be > 6.86 mg/L (TWA), since only 27.0% inhibition was observed at 6.86 mg/L (TWA). The 21d LOEC (reproduction) was determined to be 6.86 mg/L (TWA), based on biological (but not statistical) significance. The corresponding 21d NOEC (reproduction) was determined to be 1.40 mg/L (TWA).

The mortality of parent animals did not exceed 20 % at the end of the test in the control.

The mean number of live offspring produced per parent animal alive at the end of the test was >= 60 in the control.

Reference 2

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Remarks:

Summary of available data used for the endpoint assessment of the target substance

Adequacy of study:

key study

Justification for type of information:

Refer to the Category Approach Justification document provided in IUCLID6 Section 13.

Reason / purpose for cross-reference:

read-across source

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

1.4 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

reproduction

Remarks on result:

other: Source, key, 142-87-0, 2012

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

6.86 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

reproduction

Remarks on result:

other: Source, key, 142-87-0, 2012

Description of key information

 21d NOEC (Daphnia magna, reproduction) = 1.4 mg/L (measured concentration, OECD 211) 

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

1.4 mg/L

Additional information

Since no reliable chronic toxicity to aquatic invertebrates test is available for Sulfuric acids, C9-11-iso-C10 rich, alkyl esters, sodium salt, in accordance to Regulation (EC) No. 1907/2006 Annex XI, 1.5 a read-across to other structurally related substance was conducted. As the toxicity of alkyl sulfates is dependent on the carbon chain length, it is expected that the toxic effects are comparable for similar homologues.

One long-term toxicity study with Daphnia magna is available for C10AS Na (CAS 142-87-0; BASF, 2012). The test was performed according to OECD 211 guideline and GLP principles. Daphnids were exposed for 21 days under semi-static conditions to six test concentrations up to 6.86 mg/L (measured concentration). The followed parameters included mortality and reproduction of daphnids. The NOEC value based on the reproduction was determined to be 1.4 mg/L and the LOEC value as 6.86 mg/L (measured concentrations).

Due to structural and property similarities with the tested similar substances, it can be concluded that the above results apply also to Sulfuric acids, C9-11-iso-C10 rich, alkyl esters, sodium salt. The long-term NOEC values for aquatic invertebrates is 1.4 mg/L.