Ammonium iron(III) citrate

Endpoint summary

Description of key information

Biodegradation in water: screening test:

Estimation Programs Interface EPI Suite was run to predict the biodegradation potential of the test chemical in the presence of mixed populations of environmental microorganisms. The biodegradability of the substance was calculated using seven different models such as Linear Model, Non-Linear Model, Ultimate Biodegradation Timeframe, Primary Biodegradation Timeframe, MITI Linear Model, MITI Non-Linear Model and Anaerobic Model (called as Biowin 1-7, respectively) of the BIOWIN v 4.10 software. The results indicate that the test chemical was expected to be readily biodegradable in water.  

Additional information

Biodegradation in water: screening test:

Various experimental studies and predicted data have been summarized for the determination of biodegradation rate of test chemical and closest structurally and functionally similar read across chemicals which are reviewed and mention as below:

 

Estimation Programs Interface EPI Suite was run to predict the biodegradation potential of the test chemical in the presence of mixed populations of environmental microorganisms. The biodegradability of the substance was calculated using seven different models such as Linear Model, Non-Linear Model, Ultimate Biodegradation Timeframe, Primary Biodegradation Timeframe, MITI Linear Model, MITI Non-Linear Model and Anaerobic Model (called as Biowin 1-7, respectively) of the BIOWIN v 4.10 software. The results indicate that the test chemical was expected to be readily biodegradable in water.

 

Biodegradation study was conducted for 14 days for evaluating the percentage biodegradability of test chemical. Activated sludge was used as a test inoculums for the study. Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The test substance undergone 77% degradation by BOD and 100% degradation by both TOC removal and HPLC parameters. Thus, based on percentage degradation, test chemical was considered to be readily biodegradable in water.

 

 

28-days Closed Bottle test was performed according to the OECD guideline 301 D to determine the ready biodegradability of the test chemical. The test system included control, test item and reference item. Polyseed was used as inoculum in this study. The concentration of test and reference item (Sodium Benzoate) chosen for both the study was 4 mg/L, while that of inoculum was 32 ml/l. OECD mineral medium was used for the study. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % degradation was calculated using the values of BOD and ThOD for test item and reference item. The % degradation of procedure control (reference item) was also calculated using BOD & ThOD and was determined to be 70.48%. Degradation of Sodium Benzoate exceeds 46.38% on 7 days & 60.24% on 14th day. The activity of the inoculum was thus verified and the test can be considered as valid. The BOD28 value of test chemical was observed to be 0.87 mgO2/mg. ThOD was calculated as 0.88 mgO2/mg. Accordingly, the % degradation of the test item after 28 days of incubation at 20 ± 1°C according to Closed Bottle test was determined to be 98.86%. Based on the results, the test item under the test conditions was considered to be readily biodegradable in nature.

 

Thus based on the above all studies and result observations, it is concluded that the test chemical is readily biodegradable in water.