Ammonium iron(III) citrate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

of read across substance

Justification for type of information:

Data for the target chemical is summarized based on the structurally similar read across chemicals

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

WoE was prepared to determine the effect of test chemical on the mortality rate of fishes from two different studies.

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

not specified

Details on test solutions:

WoE 2: The stock solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous 1 hour stirring for achieving test concentrations of 100mg/L, respectively.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

WoE 2: TEST ORGANISM
- Common name:Zebra Fish
- Strain:Danio rerio
- Source: Unique Aqua Fish Corner
- Length at study initiation (length definition, mean, range and SD): average 1.96 cm
- Weight at study initiation (mean and range, SD): average 0.74 g
-Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod: 16 hours light and 8 hours dark
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

150.5 mg of CaCO3

Test temperature:

24.1 °C

pH:

7.1

Dissolved oxygen:

7.5 mg/l

Nominal and measured concentrations:

100 mg/l

Details on test conditions:

WoE 2: TEST SYSTEM
- Test vessel: Aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes

Reference substance (positive control):

not specified

Key result

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: WoE 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: WoE 3

Validity criteria fulfilled:

not specified

Conclusions:

Based on limit nominal concentration, experimental median lethal effect of test material on fish was observed to be >100 mg/l, as no effects concentration was observed at the limit test concentration of 100 mg/l.

Executive summary:

Data available for the test chemical have been reviewed to determine the short term toxicity of test chemical on the mortality rate of fishes. The studies are as mentioned below:

 

Study was conducted to observe the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test performed on the limit concentration of 100 mg/l. Aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes and Zebra Fishes Danio rerio were exposed to this concentration for 96 hours. A static procedure was used for the study. After 96 hours of exposure to test chemical to nominal test concentration, LC50 was calculated. Based on limit nominal concentration, experimental median lethal effect of test material on Zebra Fish Danio rerio was determine to be >100 mg/l, no effects concentration was observed at the limit test concentration of 100 mg/l. Based on the LC50 value, it can be consider that the chemical was not toxic and not classified as per the CLP classification criteria.

 

Above study was further supported by the study from authoritative database. Short term toxicity of test material was evaluated according to OECD Guideline 203 (Fish, Acute Toxicity Test). Test conducted under the static system for 96 hours. Thus based on the mortality effect on fish, the median lethal concentration LC50 value was observed to be > 100 mg/l. Based on the above effect concentration, it was considered that test substance is not toxic to fish and not classified as per CLP criteria.

 

Based on the above effect concentrations, it was considered that test substance is not toxic to fish and thus not classified as per the CLP classification criteria.

Description of key information

Based on limit nominal concentration, experimental median lethal effect of test material on fish was observed to be >100 mg/l, as no effects concentration was observed at the limit test concentration of 100 mg/l.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

Data available for the test chemical and structurally and functionally similar read across chemicals have been reviewed to determine the short term toxicity of test chemical on the mortality rate of fishes. The studies are as mentioned below:

 

Study was conducted to observe the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test performed on the limit concentration of 100 mg/l. Aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes and Zebra Fishes Danio rerio were exposed to this concentration for 96 hours. A static procedure was used for the study. After 96 hours of exposure to test chemical to nominal test concentration, LC50 was calculated. Based on limit nominal concentration, experimental median lethal effect of test material on Zebra Fish Danio rerio was determine to be >100 mg/l, no effects concentration was observed at the limit test concentration of 100 mg/l. Based on the LC50 value, it can be consider that the chemical was not toxic and not classified as per the CLP classification criteria.

 

Above study was further supported by the study from authoritative database. Short term toxicity of test material was evaluated according to OECD Guideline 203 (Fish, Acute Toxicity Test). Test conducted under the static system for 96 hours. Thus based on the mortality effect on fish, the median lethal concentration LC50 value was observed to be > 100 mg/l. Based on the above effect concentration, it was considered that test substance is not toxic to fish and not classified as per CLP criteria.

 

Based on the above effect concentrations, it was considered that test substance is not toxic to fish and thus not classified as per the CLP classification criteria.