Methanal, reaction products with 1,3-bis(aminomethyl)benzene and hydroxybenzene

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The study was conducted according to the O.E.C.D. test guideline 201 with GLP compliance.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2011

Report date:

2011

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1-[3-(aminomethyl)phenyl]methanamine; 2,4-bis[({[3-(aminomethyl)phenyl]methyl}amino)methyl]phenol; 2,6-bis[({[3-(aminomethyl)phenyl]methyl}amino)methyl]-4-[(4-hydroxyphenyl)methyl]phenol; 2-[({[3-(aminomethyl)phenyl]methyl}amino)methyl]-4-[(4-hydroxyphenyl)methyl]phenol; 2-[({[3-(aminomethyl)phenyl]methyl}amino)methyl]phenol; phenol

EC Number:

701-207-5

Cas Number:

1950616-36-0

Molecular formula:

(C8 H12 N2 . C6 H6 O . C H2 O)x

IUPAC Name:

1-[3-(aminomethyl)phenyl]methanamine; 2,4-bis[({[3-(aminomethyl)phenyl]methyl}amino)methyl]phenol; 2,6-bis[({[3-(aminomethyl)phenyl]methyl}amino)methyl]-4-[(4-hydroxyphenyl)methyl]phenol; 2-[({[3-(aminomethyl)phenyl]methyl}amino)methyl]-4-[(4-hydroxyphenyl)methyl]phenol; 2-[({[3-(aminomethyl)phenyl]methyl}amino)methyl]phenol; phenol

Test material form:

other: Liquid at room temperature.

Details on test material:

As per IUCLID5 Sections 1.1. 1.2. and 4.1.

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

For test concentrations prepared in the range- finding test, stock solutions were prepared at concentrations of 20, 100 and 200 mg/L by adding the required nominal amount of test substance in the diluent, Algal stock culture medium (Woods Hole) and mixing the solution for approximately 2 hours. For test concentrations prepared in the definitive test, stock solutions were prepared at concentrations of 40, 80 and 160 mg/L by adding the required nominal amount of test substance in the diluent, and mixing the solution for approximately 2 hours.

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

Pseudokirchneriellasubcapitata (formerly Selenastrum capricornutum), one of the freshwater alga species specified in the O.E.C.D. guideline was maintained at STILLMEADOW, Inc. Mass algal culture was initiated 3-7 + 1 days prior to dosing. The algae were maintained in Algal stock culture medium (Woods Hole) with EDTA up to 0.300 mg/L, at a temperature of 22-25°C with Continuous lighting approximately 422 fi. c. and continuous aeration.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Post exposure observation period:

None

Test conditions

Hardness:

No data

Test temperature:

22-25°C

pH:

7.9-10

Dissolved oxygen:

Saturated

Salinity:

No data

Nominal and measured concentrations:

5, 10, 20, 40 and 80 mg/L nominal.

Details on test conditions:

Treatment was conducted in 250-mL sterile Erlenmeyer flasks with stoppers and aeration tubing and 50,000 cells/mL. There were three flasks for each test substance concentration and six flasks for the untreated control. A positive control group consisted of three flasks treated with 10 mg/L zinc chloride. The flasks were set on a shaker table for constant agitation at approximately 100 cycles/minute. At 24, 48 and 72 hours following dosing, observations of inhibition were made for each test container by measuring the mean cell density with a hemacytometer. The temperature was recorded daily, and the pH of each container was recorded at study termination.

Reference substance (positive control):

yes

Remarks:

Zinc chloride at 10 mg/L.

Results and discussion

Effect concentrations

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

ca. 20.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

cell number

Remarks on result:

other: 17.6-24.5 mg/L.

Details on results:

Little or no increase in cell number was observed at the test substance concentrations of 40 and 80 mg/L throught the study. The relative precent survival at 20 mg/L was 47.9 at 72 hr. The percent survial of the 10 mg/L concentration was 85.9% ay 72 hr and is considered the NOEC.

Results with reference substance (positive control):

There was no increase in algal cell number with the positive control zinc chloride.

Reported statistics and error estimates:

The NOEC and EC50 values were determined by a commercially available statistical program (ToxCalc Version 5.OTM). The Maximum Likelihood-Logit model was selected for the determination in this case. The 95% confidence Limits on the EC50 value were 17.6-24.5 mg/L.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The estimated EC50 value for the test substance was 20.4 mg/L with 95% confidence Limits of 17.6-24.5 mg/L. This EC50 value is the lowest of the three aquatic species and is therefore the most appropriate value for derivation of PNEC values.

Executive summary:

The acute toxicity of the test substance, Formaldehyde, polymer with 1,3 -benzenedimethanamine and phenol to algae was determined in an O.E.C.D. test guideline 201 study. The estimated EC50 value for the test substance was 20.4 mg/L with 95% confidence Limits of 17.6-24.5 mg/L following 72 hr of exposure. .