Hexasodium 4,4'-[1,4-phenylenebis[imino(6-chloro-1,3,5-triazine-4,2-diyl)imino]]bis[5-hydroxy-6-[(2-sulphonatophenyl)azo]naphthalene-2,7-disulphonate]

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

22 November 2017 to 28 November 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.1075 (Freshwater and Saltwater Fish Acute Toxicity Test)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

at 0 and 96 h from controls and at 141 mg/L

Vehicle:

no

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Danio rerio
- Strain: HAMILTON-BUCHANAN
- Source: original animals were obtained from Umweltbundesamt (UBA) and used for breeding and production of eggs.
- Age at study initiation: sexually immature young fish
- Length at study initiation: 2 ± 1 cm
- Maintenance of the brood fish:
Vessels polyethylene aquaria
Medium chlorine-free tap water
Feeding three times a day with warm-water fish food , totalling about 1-2 % of body weight per day
Photo period 12/12 hours, using neon tubes
Temperature 23 - 26°C

ACCLIMATION : no data

FEEDING DURING TEST :none

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

0.71 mmol/L

Test temperature:

22.5 - 24.2 ˚C

pH:

7.8

Dissolved oxygen:

7.5-8.7 mg/L

Nominal and measured concentrations:

nominal: 0 and 141 (100 a.i.) mg/L
measured: ND, 97-113% of nominal

Details on test conditions:

TEST SYSTEM
- Test vessel: glass aquaria, maximal volume 10 L
- Fill volume: 7 L
- Aeration: yes with glass tubes
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: stated to be < 1 g/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water (analytical report included)

OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 12/12 hours using neon tubes

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality and abnormal behaviour every 24 hours

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 141 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No abnormal behaviour of the surviving fish was observed.

Sublethal observations / clinical signs:

Mortality

Nominal Conc. in mg/L	Total number of fish used	Number of dead fish
		24 h	48 h	72 h	96 h
Blank control	7	0	0	0	0
141	7	0	0	0	0

Validity criteria fulfilled:

yes

Conclusions:

The LC50 in fish of the substance is > 141 mg/L (100 mg/L as a.i.)

Executive summary:

The toxicity against Danio rerio was tested using a static test design.

The study was performed using one concentration containing 141 mg/L test item (corresponding to 100 mg/L main constituent). The concentration showed no mortality.

At the start and at the end of the test, the content of the test item in the test solutions was determined using HPLC. The correlation between nominal and measured concentration was good (97 % and 113 %). Therefore, the determination of the results was based on the nominal concentration.

The 96h-EC50 was > 141 mg/L test item resp. 100 mg/L main constituent.

 

Description of key information

The toxicity against Danio rerio was tested using a static test design as a limit test at141 mg/L test item (corresponding to 100 mg/L main constituent). Measured concentrations were 97 -113% of nominal. No mortality was observed and the 96h-EC50 was > 141 mg/L (> 100 mg/L for the active ingredient) (Laus 2017).

In a test according to OECD 203, 10 fish were exposed to 0, 1, 10, 50 and 100 mg/L of a 50% formulation of the substance during 96 hours. At 100 mg/L 80% mortality was observed. At all other concentrations no mortality or behavioural abnormalities were observed. The LC50 is 74.8 mg/L. The publication contains only limited information and it is not clear whether other components of the formulation may have influenced this result (Darsana 2015).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

141 mg/L