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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Description of key information

EC50 (48 h) > 100 mg/l (nominal)

Key value for chemical safety assessment

Additional information

There are no information about the short-term toxicity potential to aquatic invertebrates of Acid Red 336, thus the available data on structural analogous Similar Substance 02 have been taken into account (details and explanations are included in the report attached to the IUCLID section 13).

Similar Substance 02 was assessed using Daphnia magna, according to the OECD guideline 202. A static limit test with concentrations of 0 and 100 mg/l was performed. Analytical samples taken at 0 hours (initial value) from fresh test solutions and after 48 hours from aged test solutions were analysed from control and 100 mg/l: the initial measured content at 100 mg/l was 97 % of nominal and the aged measured content was 95 % of nominal. Therefore, the toxicological endpoints were evaluated using nominal concentrations. After the 48 hours exposure period, no immobilisation was recorded at 100 mg/l.