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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Range Finding Study from April 30 to May 04, 1991; main experiment in May from 07 to 11, 1991
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
not specified
Details on test solutions:
Direct dispersion in water.
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
TEST ORGANISM
- Common name: rainbow trout.
- Source: Parkwood Trout Farm, Kent, U.K.
- Length at study initiation: 4.7 ± 0.3 cm
- Weight at study initiation: 1.51 ± 0.31 g

ACCLIMATION
- Medication: none
- Health during acclimation: mortalities lower than 7 % in the 7 days prior to the start of the test.
Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Test temperature:
14 °C
Nominal and measured concentrations:
Nominal 10 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel: 20 litres
- Aeration: yes
- Renewal rate of test solution: daily.
- No. of vessels per concentration: 2 replicates.
- No. of vessels per control: 1 replicate.
- Biomass loading rate: 0.58 g bodyweight/litre.

TEST MEDIUM / WATER PARAMETERS
- Source of dilution water: dechlorinated tap water (sodium thiosulphate).
- Hardness: approximately 50 mg/l as CaC03.

TEST CONCENTRATIONS
Range finding study
- Test concentrations: 1.0, 10 and 100 mg/l
- No organism per concentration: 2
- Results used to determine the conditions for the definitive study: 100 % of fish died at the highest tested concentration.
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
During the definitive study, no deaths occurred.
Sublethal observations / clinical signs:

Definitive study

Vessel Cumulative mortality (initial population = 10)
24 hrs 48 hrs 72 hrs 96 hrs
Control 0 0 0 0
Test item 10 mg/l 0 0 0 0
Test item 10 mg/l 0 0 0 0

Range Finding study

Vessel Cumulative mortality (initial population = 2)
24 hrs 48 hrs 72 hrs 96 hrs
Control 0 0 0 0
Test item 1 mg/l 0 0 0 0
Test item 10 mg/l 0 0 0 0
Test item 100 mg/l 2 2 2 2
Conclusions:
LC50 (96h) > 10 mg/l (nominal)
Executive summary:

The acute lethal toxicity (LC50) of test item to rainbow trout was investigated under semi-static exposure conditions, over a period of 96 hours. The nominal concentration for the definitive test was established on the basis of the range-finding study , which was conducted under static conditions: the substance was tested at 1.0, 10 and 100 mg/l; 100 % of mortality was recorded at the highested tested concentration. Thus, the limit test was conducted at 10 mg/l.

During the definitive test, no deaths occurred either in the test vessels, neither in the control one.

Conclusion

LC50 (96h) > 10 mg/l (nominal)

Description of key information

LC50 (96h): 10 - 100 mg/l (nominal)

Key value for chemical safety assessment

Additional information

The acute lethal toxicity (LC50) of test item to rainbow trout was investigated under semi-static exposure conditions, over a period of 96 hours. The nominal concentration for the definitive test was established on the basis of the range-finding study , which was conducted under static conditions: the substance was tested at 1.0, 10 and 100 mg/l; 100 % of mortality was recorded at the highested tested concentration. Thus, the limit test was conducted at 10 mg/l. Thus, the limit test was conducted at 10 mg/l. During the definitive test, no deaths occurred either in the test vessels, neither in the control one.

In addition, an old test performed on the substance under registration is also available. The indicated LC50 value at 48 hours is 60 mg/l; the LC0 was indicated to be 20 mg/l, while the LC100 was idicated at 100 mg/l. There are no details on testing method and procedures followed; only the sheet of results is available, thus reliability cannot be assigned and the data has not been used for classification.

Data on the Acid Violet 048 acid form has also been taken into consideration, in order to clarify the potential toxicity of the substance to fish. The acute lethal toxicity (LC50) of test item to rainbow trout was investigated under semi-static exposure conditions, over a period of 96 hours. The nominal concentration for the definitive test was established on the basis of the range-finding studis. A first limit test was carried out at 100 mg/l; however, in the test progressive mortalities occurrred due to the daily renewel of test media as opposed to the static conditions under which the range-finding experiment was carried out. Therefore, the limit test was repeated at 10 mg/l to achieve a true No-Effect Level. During the definitive test conducted at the limit concentration of 10 mg/l, no deaths occurred either in the test vessels, neither in the control one.