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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Study period:
January 22 to March 22, 1991
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Justification for type of information:
The read across approach is detailed into the document attached into the "information panel" of the Aquatic Toxicity endpoint summary.
Reason / purpose for cross-reference:
read-across source
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes
Details on test solutions:
The substance was direct dispersed in water.
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Strain: laboratory culture originating from a strain supplied by I.R.CH.A., France.
- Breeding: testing laboratoyr, cultures maintained at 21°C in polypropylene vessels containing two litres of dechlorinated and aged tap water. Culture conditions ensure that reproduction is by parthenogenesis.
- Age of parental stock: gravid adults were isolated 24 hours prior to initiation of the test. Young daphnids produced overnight were used for testing.
- Feed: cultures were fed daily with a suspension of mixed algae (predominantly Chlorella spp.).
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Test temperature:
Start: 21 °C
End: 21 °C
pH:
Start: 7.4
End: 7.2
Dissolved oxygen:
Start: 8.7 mg O2/l
Start: 8.1 mg O2/l
Nominal and measured concentrations:
5.0, 9.0, 16, 28, 50, 90, 160, 280 and 500 mg active ingredient/l
Details on test conditions:
TEST SYSTEM
- Daphnia loading: according to test protocol.

TEST MEDIUM / WATER PARAMETERS
- Water quality characteristics: according to test protocol.
Reference substance (positive control):
yes
Remarks:
K2Cr2O7
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
140 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality
Details on results:
EC0 (48h): 16 mg a.i./l (nominal)
EC100 (48h): 500 mg a.i./l (nominal)
Results with reference substance (positive control):
EC50 (24h): 1.5 mg/l

Cumulative immobilisation of daphnids (%) test item

Concentration (mg a.i./l) 24 hrs 48 hrs
Control 0 0
Test item 5.0 0 0
Test item 9.0 0 0
Test item 16 0 0
Test item 28 5 5
Test item 50 5 15
Test item 90 10 20
Test item 160 20 60
Test item 280 30 70
Test item 500 60 100

Immobilisation rate of daphnids (%) reference substance

Concentration (mg a.i./l) 24 hrs 48 hrs
Control 0 0
Reference substance 0.10 0 0
Reference substance 0.18 0 0
Reference substance 0.32 0 0
Reference substance 0.56 5 5
Reference substance 1.0 5 15
Reference substance 1.8 10 20
Reference substance 3.2 20 60
Reference substance 5.6 30 70
Reference substance 10 60 100
Conclusions:
EC50 (48h): 140 mg a.i./l (nominal)
Executive summary:

The acute toxicity of substance to Daphnia magna was investigated under static exposure conditions over a period of 48 hours, according to the OECD testing guideline. The following concentrations were tested: 5.0, 9.0, 16, 28, 50, 90, 160, 280 and 500 mg active ingredient/l.

Potassium dichromate was also tested as reference substance.

The maximum concentration causing no immobilization after 48 hours was 16 mg a.i./l (nominal); on the contrary, at the concentration of 500 mg a.i./l all the test organisms resulted to be immobilized. The EC50 at 48 hours was determied to be 140 mg a.i./l.

Conclusion

EC50 (48h): 140 mg a.i./l (nominal)

Description of key information

EC50 (48h): 140 mg a.i./l (nominal)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
140 mg/L

Additional information

There are no information about the short-term potential toxicity of Acid Violet 048 to acquatic invertebrates, thus the available information on the structural analogous Similar Substance 01 has been taken into consideration; the read across approach can be considered as appropriate and suitable to assess the property under investigation (details about the approach are reported into the document attached into the "information panel" of the Aquatic Toxicity endpoint summary).

The acute toxicity of Similar Substance 01 to Daphnia magna was investigated under static exposure conditions over a period of 48 hours, according to the OECD testing guideline 202. The maximum concentration causing no immobilization after 48 hours was 16 mg a.i./l (nominal); on the contrary, at the concentration of 500 mg a.i./l all the test organisms resulted to be immobilized. The EC50 at 48 hours was determied to be 140 mg a.i./l.