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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2005
Report date:
2005

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
Reference substance 001
EC Number:
607-771-8
Cas Number:
25655-41-8
Molecular formula:
C6H9I2NO
Details on test material:
- Name of test material (as cited in study report): PVP-lod 30/06
- Analytical purity: 97.2 g/100 g
- Lot/batch No.: Lot: G2295BPZO

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution was prepared by stirring the test substance in M4 medium for about 10 minutes at 20 ± 2 °C . The concentration of the stock solution was 10. 1 mg/2l .The test solutions were prepared by diluting the stock solution.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Source: originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.
- Age at study initiation (mean and range, SD): 2 - 24 h

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
2.42 mmol/l
Test temperature:
20.3 - 20.6 °C
pH:
8.0 - 8.3
Dissolved oxygen:
9.1 - 9.5 mg/l
Nominal and measured concentrations:
Nominal test substance concentrations:
control, 0.156, 0.313, 0.625, 1.25, 2.5, 5 mg/l

Mean measured concentrations:
0.190, 0.369, 0.731, 1.50, 2.90, 5.85 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel: flat bottom glass tubes (20 ml)
- Fill volume: 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.5 animals/ml

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4 medium according to OECD guideline
- Alkalinity up to pH 4.3: 0.8 - 1.0 mmol/l
- Ca/mg ratio: 4:1
- Conductivity: 590 µS/cm


OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime
- Light intensity: Artificial light, type warm white (OSRAM L58 W31), about 1 - 8 µE/(m²s) at a wave length of 400 - 750 nrn

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 0, 24 and 48 hours

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
2.9 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
3.23 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
5.85 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
2.79 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Applicant's summary and conclusion

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