Reaction mass of iodine and 2-Pyrrolidinone, 1-ethenyl-, homopolymer

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2005

Report date:

2005

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Reference substance 001

EC Number:

607-771-8

Cas Number:

25655-41-8

Molecular formula:

C6H9I2NO

Details on test material:

- Name of test material (as cited in study report): PVP-lod 30/06
- Analytical purity: 97.2 g/100 g
- Lot/batch No.: Lot: G2295BPZO

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution was prepared by stirring the test substance in M4 medium for about 10 minutes at 20 ± 2 °C . The concentration of the stock solution was 10. 1 mg/2l .The test solutions were prepared by diluting the stock solution.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.
- Age at study initiation (mean and range, SD): 2 - 24 h

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.42 mmol/l

Test temperature:

20.3 - 20.6 °C

pH:

8.0 - 8.3

Dissolved oxygen:

9.1 - 9.5 mg/l

Nominal and measured concentrations:

Nominal test substance concentrations:
control, 0.156, 0.313, 0.625, 1.25, 2.5, 5 mg/l

Mean measured concentrations:
0.190, 0.369, 0.731, 1.50, 2.90, 5.85 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: flat bottom glass tubes (20 ml)
- Fill volume: 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.5 animals/ml

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4 medium according to OECD guideline
- Alkalinity up to pH 4.3: 0.8 - 1.0 mmol/l
- Ca/mg ratio: 4:1
- Conductivity: 590 µS/cm


OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime
- Light intensity: Artificial light, type warm white (OSRAM L58 W31), about 1 - 8 µE/(m²s) at a wave length of 400 - 750 nrn

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 0, 24 and 48 hours

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

2.9 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

3.23 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

5.85 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

2.79 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility