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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

Under the test condition the test item is classified as not readily biodegradable in the 10 day window and after 28 days.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

The ready biodegradability was determined with a non adapted activated sludge for the test item Hydroxyethyl-p-phenylenediamine sulfate (Betoxol II) over a test period of 28 days in the Modified Sturm Test. The test item was tested in a concentration of 30mg/L in duplicates, corresponding to a carbon content (TOC) of 11.5mgC/L in the test vessels. The biodegradation of the test item was followed by titrimetric analyses of the quantity of CO2 which was produced by the respiration of bacteria. The degradation was finished on day 28 by acidification, the last titration was performed on day 29, after the soluble CO2 was turned out over a period of 24 hours. The CO2 production was calculated as the percentage of total CO2 that the test item could have theoretically produced based on carbon content. Biodegradation is therefore expressed as percentage ThCO2 and was calculated for each titration of CO2. In order to check the activity of the test system sodium acetate was used as a functional control. The percentage degradation of the functional control reached the pass level of >60% after 10 days. After 14 days a degradation rate of 70% was reached. The validity criterion of the guideline is fulfilled. in the toxicity control containing both test and reference item a biodegradation rate of 36% occurred within 14 days. At this point of time the plateau was reached. The biodegradation of the reference item was not inhibited by the test item in the toxicity control. The 10% level (beginning of biodegradation) was not reached, neither in the 10 day window nor after 28 days.The biodegradation remained at a level of 0 -3% after 28 days. The test item must be regarded to be not readily biodegradable in the 10 day window and after 28 days. The validity criteria according to the guideline are fulfilled.