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EC number: 235-985-8 | CAS number: 13080-86-9
Bioaccumulation: aquatic / sediment
Administrative data
- Endpoint:
- bioaccumulation in aquatic species: fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 03 Sep - 28 Nov 1985
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 985
- Report date:
- 1985
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: “Bioconcentration test of chemical substances in fish and shellfish” specified in the Test Method Relating to New Chemical Substances (Kanpogyo No.5, Yakuhatsu No.615, 49 Kikyoku No.392
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 4,4'-[isopropylidenebis(4,1-phenyleneoxy)]dianiline
- EC Number:
- 235-985-8
- EC Name:
- 4,4'-[isopropylidenebis(4,1-phenyleneoxy)]dianiline
- Cas Number:
- 13080-86-9
- Molecular formula:
- C27H26N2O2
- IUPAC Name:
- 4,4'-[isopropylidenebis(4,1-phenyleneoxy)]dianiline
Constituent 1
- Radiolabelling:
- no
Sampling and analysis
- Details on sampling:
- - Sampling intervals/frequency for test organisms: 3 fish were tested from the 1st and 2nd concentration groups, 2 fish were used for each analysis
- Sample storage conditions before analysis: 1 fish with aluminum foil and kept frozen in the plastic bag
- Details on sampling and analysis of test organisms and test media samples: See detailed analytical method in section "Any other information on materials and methods incl tables".
Test solutions
- Vehicle:
- yes
- Remarks:
- dimethyl sulfoxide
- Details on preparation of test solutions, spiked fish food or sediment:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: 1.00 g of the test substance and 20.0 g of the dispersant (hydrogenated castor oil HCO-40) were dissolved in dimethyl sulfoxide to obtain the constant volume of 100 mL. This solution was diluted with water to obtain the constant volume of 5.0 L to prepare 200 ppm (W/V) of stock solution.
- Controls: yes
Test organisms
- Test organisms (species):
- Oryzias latipes
- Details on test organisms:
- TEST ORGANISM
- Common name: Carp
- Strain: Oryzias latipes
- Length at study initiation (length definition, mean, range and SD): 10.3 ± 0.2 cm (average)
- Weight at study initiation (mean and range, SD): 0.29 g (average); 24.5 ± 2.7 g
- Lipid content at test initiation (mean and range, SD): 3.8 ± 0.4%
ACCLIMATION
- Acclimation period: 25°C for 20 days
Quarantine:
- Quarantine conditions: Fish were treated with a medicated bath of Terramycin solution (100 ppm)
- Quarantine duration: 24 hours under static conditions (2 times).
Study design
- Route of exposure:
- aqueous
- Test type:
- flow-through
- Water / sediment media type:
- natural water: freshwater
- Total exposure / uptake duration:
- 8 wk
Test conditions
- Test temperature:
- 25.0 ± 1.0 °C (24.5±1.0°C)
- Details on test conditions:
- TEST SYSTEM
Test vessel:
- Type: open
- Material, size, headspace, fill volume: Glass aquaria, volume 100 L
- Type of flow-through: Water flow 300 mL/min (432 L/day) - Nominal and measured concentrations:
- 1st concentration group: 0.5 ppm
2nd concentration group: 0.05 ppm - Reference substance (positive control):
- not specified
Results and discussion
Lipid content
- Remarks on result:
- other:
- Remarks:
- no information were given in the report
Bioaccumulation factoropen allclose all
- Conc. / dose:
- 0.5 other: ppm
- Type:
- BCF
- Value:
- > 303 - < 327 dimensionless
- Time of plateau:
- 56 d
- Remarks on result:
- other: 56 days after the test
- Remarks:
- a mean of 315
- Conc. / dose:
- 0.05 other: ppm
- Type:
- BCF
- Value:
- > 492 - < 518 dimensionless
- Time of plateau:
- 56 d
- Remarks on result:
- other: 56 days after the test
- Remarks:
- a mean of 505
Depuration
- Remarks on result:
- other: no information were given in the report
Rate constants
- Remarks on result:
- other:
- Remarks:
- no information were given in the report
Any other information on results incl. tables
Test results
Number of days after the test |
1st concentration group |
2nd concentration group |
||
A B |
BCF (bioconcentration factor) |
A’ B’ |
BCF (bioconcentration factor) |
|
3 |
A |
98 |
A’ |
258 |
B |
96 |
B’ |
200 |
|
7 |
A |
237 |
A’ |
390 |
B |
163 |
B’ |
548 |
|
14 |
A |
331 |
A’ |
550 |
B |
272 |
B’ |
612 |
|
21 |
A |
350 |
A’ |
458 |
B |
322 |
B’ |
487 |
|
28 |
A |
323 |
A’ |
462 |
B |
299 |
B’ |
542 |
|
42 |
A |
293 |
A’ |
580 |
B |
269 |
B’ |
504 |
|
56 |
A |
303 |
A’ |
518 |
B |
327 |
B’ |
492 |
|
(Note 1) A, B in the 1st concentration group and A' and B' in the 2nd concentration group are the marks attached to the sample fish at the time of measurement of accumulation.
Applicant's summary and conclusion
- Conclusions:
- The BCF was determined to be 505 (mean of 492 and 518) after 56 days at 0.05 ppb.
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