Registration Dossier
Registration Dossier
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EC number: 250-005-9 | CAS number: 30030-25-2
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
Toxicity to aquatic algae and cyanobacteria, OECD TG 201, Pseudokirchneriella subcapitata, static, 72 hours, results: ErC50 (72h) = 0.489 mg/L, ErC10 (72h) = 0.364 mg/L
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 0.489 mg/L
- EC10 or NOEC for freshwater algae:
- 0.364 mg/L
Additional information
Toxicity to aquqatic algae and cyanobacteria
The purpose of this GLP study was to assess the potential effects of vinylbenzyl chloride to the freshwater green alga, Pseudokirchneriella subcapitata according to OECD Guideline 201.
The study was performed for 72 hours with target nominal test concentrations of 0 (AAP medium control), 6.25, 12.5, 25.0, 50.0 and 100% of a 1000 mg vinylbenzyl chloride/L (AAP medium) nominally-dosed saturated solution. Cell density was determined at approximately 24, 48 and 72 hours (±1 hour from test initiation). Temperatures during the exposure period ranged from 22-24°C. The pH ranged from 6.4-7.8 and the light intensity ranged from 4510-5450 lux.
Test solutions were analyzed for vinylbenzyl chloride concentrations at test initiation, following 24 and 48 hours of exposure and at exposure termination by gas chromatography with electron capture detection (GC/ECD). The validity criteria were fulfilled.
The toxicity values for Pseudokirchneriella subcapitata exposed to vinylbenzyl chloride over a 72-hour static exposure period and based on geometric mean measured concentrations were as follows:
Cell yield
72-hour EyC50: 0.312 mg/L (95% C.I. = 0.284 - 0.342 mg/L)
72-hour EyC20: 0.249 mg/L (95% C.I. = 0.219 - 0.283 mg/L)
72-hour EyC10: 0.221 mg/L (95% C.I. = 0.190 - 0.258 mg/L)
72-hour NOEC: 0.0704 mg/L
Growth rate
72-hour ErC50: 0.489 mg/L (95% C.I. = 0.424 - 0.563 mg/L)
72-hour ErC20: 0.403 mg/L (95% C.I. = 0.349 - 0.464 mg/L)
72-hour ErC10: 0.364 mg/L (95% C.I. = 0.316 - 0.419 mg/L)
72-hour NOEC: 0.128 mg/L
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