Triethyl O-acetylcitrate

Endpoint summary

Key value for chemical safety assessment

Skin irritation / corrosion

Link to relevant study records

Reference

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

presumably 1974

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Only results given. Publication has lack of methodical details

Qualifier:

no guideline followed

Principles of method if other than guideline:

1.) 3 male albino rabbits were used. The abdomen were closely clipped and 1 mL/kg bw was placed on the intact skin daily for 4 days. The animals were observed daily and for a period of 36 h after the last application.

2.) 3 male albino rabbits were used. 1 mL/kg was placed on the closely clipped abdomen for a total of 18 applications (6 d per week). Animals were observed daily and for a period of two weeks following the last application.

GLP compliance:

not specified

Species:

rabbit

Strain:

other: Albino

Details on test animals or test system and environmental conditions:

no further data

Type of coverage:

not specified

Preparation of test site:

other: intact / abraded

Vehicle:

not specified

Controls:

not specified

Amount / concentration applied:

1 mL/kg bw (ca. 1000 mg/kg)

Duration of treatment / exposure:

4 - 18 applications

Observation period:

36 h to 2 weeks after last application

Number of animals:

2-3

Details on study design:

see above

Remarks on result:

no indication of irritation

Irritant / corrosive response data:

No irritant effects noted

Individual scores were not given. Examination time points: 36 h to 2 weeks after last application.

The skin irritation potential of Acetyl Triethyl Citrate was evaluated using three male albino rabbits. The test substance (1 cc/kg body weight) was inuncted onto intact abdominal skin that had been clipped free of hair. This procedure was repeated daily for 4 days. The animals were observed daily for up to 36 hours after the last application. Acetyl Triethyl Citrate did not induce skin irritation in any of the three rabbits tested .

In another study, Acetyl Triethyl Citrate (1 cc/kg body weight) was inuncted onto intact abdominal skin of each of three male albino rabbits for a total of 18 applications (6 days/week). The animals were then observed over a period of 2 weeks. Body weights were normal throughout the study. Skin irritation was not observed in any of the animals .

Interpretation of results:

GHS criteria not met

Conclusions:

ATEC was not skin irritating. Classification criteria are not meet.

Executive summary:

In all of the 2 trials there was no indication for skin irritation.

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed (not irritating)

Eye irritation

Link to relevant study records

Reference

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

presumably 1974

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Methodical details are missing

Qualifier:

no guideline followed

Principles of method if other than guideline:

3 male albino rabbits were used. 0.1 mL was instilled once into the left conjunctival sac, while the right eyes were used as controls. Observations were made at 20 min and at 3 – 72 h after application.

GLP compliance:

not specified

Species:

rabbit

Strain:

other: Albino

Details on test animals or tissues and environmental conditions:

no data

Vehicle:

not specified

Controls:

other: right eyes were used as controls

Amount / concentration applied:

0.1 mL

Duration of treatment / exposure:

single application

Observation period (in vivo):

Observations were made at 20 min and at 3 – 72 h after application.

Number of animals or in vitro replicates:

3 m

Details on study design:

SCORING SYSTEM: no data

Remarks on result:

other: Transient erythema (slight to moderate) of the palpebral conjunctivae was observed in two of the three rabbits. After 24 hours, the erythema was described as negligible.

Transient erythema (slight to moderate) of the palpebral conjunctivae was observed in two of the three rabbits. After 24 hours, the erythema was described as negligible.

Interpretation of results:

GHS criteria not met

Conclusions:

ATEC was slightly eye irritating. However, classification criteria are not meet.

Executive summary:

3 male albino rabbits were used. 0.1 mL was instilled once into the left conjunctival sac, while the right eyes were used as controls. Observations were made at 20 min and at 3 – 72 h after application. Transient erythema (slight to moderate) of the palpebral conjunctivae was observed in two of the three rabbits. After 24 hours, the erythema was described as negligible.

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed (not irritating)

Respiratory irritation

Endpoint conclusion

Endpoint conclusion:

no study available

Justification for classification or non-classification

Based on the results of the available experiments, classification criteria are not met.