4-[3-(4-chlorophenyl)-4,5-dihydro-1H-pyrazol-1-yl]benzenesulphonamide

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

November 10th, 1992

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

no

Details on test solutions:

Due to low solubility, no stock solution was used. The test material was weighed directly into the vessels of the batches.

Test organisms (species):

activated sludge of a predominantly domestic sewage

Details on inoculum:

- Source: municipal wastewater treatment plant Frankfurt / Sindlingen.
- Amount used, activated sludge: 1600 mg/l dry weight.
- Pretreatment: as specified in the testing guideline.
- Acclimation state: tot acclimated in the laboratory to the test substance and the reference substance.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Test temperature:

20 ± 2 °C

pH:

7.2 ± 2, before the measurement of the carbon content.

Nominal and measured concentrations:

In the range of 500 - 1000 mg/l

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Duration:

3 h

Dose descriptor:

IC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Details on results:

IC20 (3h) > 1000 mg/l
IC80 (3h) > 1000 mg/l

Conclusions:

IC50 (3h) > 1000 mg/l

Executive summary:

The inhibltory effect of test item on waste-water bacteria was investigated in a respiration test, according to the OECD guideline 209. Due to low solubility, no stock solution was used; the test material was weighed directly into the vessels of the batches. the tested concentrations were in the range of 500 - 1000 mg/l. 3,5-dichlorophenol was used as reference substance.

The IC20, IC50 and IC80 after 3 hours of exposure resulted to be higher than 1000 mg/l.

Conclusion

IC50 (3h) > 1000 mg/l

Description of key information

IC50 (3h) > 1000 mg/l

Additional information

The inhibltory effect of test item on waste-water bacteria was investigated in a respiration test, according to the OECD guideline 209. Due to low solubility, no stock solution was used; the test material was weighed directly into the vessels of the batches.the tested concentrations were in the range of 500 - 1000 mg/l. 3,5-dichlorophenol was used as reference substance. The IC20, IC50 and IC80 after 3 hours of exposure resulted to be higher than 1000 mg/l.