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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:
toxicity to soil macroorganisms except arthropods: short-term
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1996-02-29 to 1996-03-14
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
The study was conducted according to an EU method, and in compliance with GLP, however no analytical monitoring was undertaken.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1996
Report Date:
1996

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
EU Method C.8 (Toxicity for Earthworms: Artificial Soil Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Physical state: colourless liquid

- Stability under test conditions: substance is susceptible to hydrolysis

- Manufacture date: October 1994

- Stability of sample: >1 year

Sampling and analysis

Analytical monitoring:
no

Test substrate

Vehicle:
no

Test organisms

Test organisms (species):
Eisenia fetida
Animal group:
annelids
Details on test organisms:
TEST ORGANISM

- Common name: Earthworm, Eisenia foetida foetida

- Source: Eisenia GmbH und Co, Wiesbaden, Germany

- Age at test initiation (mean and range, SD): 2 months

- Weight at test initiation (mean and range, SD): 300 - 600 mg

Study design

Study type:
laboratory study
Substrate type:
other: quartz sand
Limit test:
yes
Total exposure duration:
14 d

Test conditions

Test temperature:
20 +/-2 ºC
pH:
5.7
Moisture:
40%
Details on test conditions:
TEST SYSTEM

- No. of organisms per container (treatment): 10

- No. of replicates per treatment group: 4

- No. of replicates per control: 4

SOURCE AND PROPERTIES OF SUBSTRATE (if soil)

- Composition (if artificial substrate): Quartz sand

OTHER TEST CONDITIONS

- Photoperiod: Daylight

- Light intensity: ca. 400 Lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality after 7 and 14 days exposure

VEHICLE CONTROL PERFORMED: no

TEST CONCENTRATIONS

- Limit test at 1000 mg/kg
Nominal and measured concentrations:
Nominal: 1000 mg/kg
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
> 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
14 d
Dose descriptor:
NOEC
Effect conc.:
>= 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- Mortality at end of exposure period: 0
Reported statistics and error estimates:
No toxic effects on survival and growth were observed in the test and therefore statistical analysis of the results was not required.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
A reliable 14-day LC50 of >1000 mg/kg dry weight has been reported for the test substance to Eisenia foetida. A NOEC of ≥1000 mg/kg dry weight was also determined in the test.