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Toxicological information

Skin irritation / corrosion

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Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1986
Report date:
1986

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
2,6-dichlorobenzyl chloride
EC Number:
217-940-4
EC Name:
2,6-dichlorobenzyl chloride
Cas Number:
2014-83-7
Molecular formula:
C7H5Cl3
IUPAC Name:
2,6-dichlorobenzyl chloride

Test animals

Species:
rabbit
Strain:
New Zealand White
Details on test animals or test system and environmental conditions:
- Source: Hoechst AG
- Number of animals: 3 per duration of application
- Age: 3 - 5 months
- Weight: 2.1 - 3.2 kg
- Keeping: Single cages, fully air-conditioned, 20 +- 3 °C, 50 +- 20 % rh, 12 h light per day
- Feed: Altromin 22123 ad libitum, 15 g hay per day, water ad libitum

Test system

Type of coverage:
semiocclusive
Preparation of test site:
shaved
Vehicle:
unchanged (no vehicle)
Amount / concentration applied:
0,5 ml per animal
Duration of treatment / exposure:
4 hours or 3 minutes
Observation period:
30 - 60 minutes, 24, 48, 72 hours after removal. Signs were visible after 72 hours, so additional rating after 7 and 14 days.
Number of animals:
3 per application time
Details on study design:
TEST SITE
- Area of exposure: 2.5 x2.5 cm² (6.25 cm²)
- Type of wrap if used: pulp, additionally covered with semiocclusive tape

REMOVAL OF TEST SUBSTANCE
- Washing (if done): tap water
- Time after start of exposure: 3 min / 4 h

SCORING SYSTEM: according to OECD TG 404

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
edema score
Remarks:
4 hour application
Basis:
animal #1
Time point:
24/48/72 h
Score:
2.3
Max. score:
4
Reversibility:
fully reversible within: 7 d
Irritation parameter:
edema score
Remarks:
4 hour application
Basis:
animal #2
Time point:
24/48/72 h
Score:
2
Max. score:
4
Reversibility:
fully reversible within: 14 d
Irritation parameter:
edema score
Remarks:
4 hour application
Basis:
animal #3
Time point:
24/48/72 h
Score:
2
Max. score:
4
Reversibility:
fully reversible within: 14 d
Irritation parameter:
erythema score
Remarks:
4 hour application
Basis:
animal #1
Time point:
24/48/72 h
Score:
2.7
Max. score:
4
Reversibility:
fully reversible within: 14 d
Irritation parameter:
erythema score
Remarks:
4 hour application
Basis:
animal #2
Time point:
24/48/72 h
Score:
2
Max. score:
4
Reversibility:
not reversible
Irritation parameter:
erythema score
Remarks:
4 hour application
Basis:
animal #3
Time point:
24/48/72 h
Score:
2
Max. score:
4
Reversibility:
not reversible
Irritant / corrosive response data:
White skin after 24, 48, 72 hours, whole flank reddened after 24 hours, hardened skin (2/3), cracked skin (3/3), bulged skin (2/3), scab formation (1/3) after 7 days, dry skin (1/3), parchmenty skin, scar formation, scab formation, shedding in one animal each after 14 days.

Applicant's summary and conclusion

Interpretation of results:
Category 1C (corrosive) based on GHS criteria
Conclusions:
According to test results corrosive to skin.
Executive summary:

2,6-dichlorobenzyl chloride was tested for skin irritating or skin corrosive properties according to OECD 404. Following this protocol 500 mg of the test substance were applied to the shaved skin of 6 New Zealands White rabbits. After 3 minutes or 4 h exposure time the semiocclusive patch was removed. Residual test substance was removed with lukewarm tap water. Animals were examined for signs of skin irritation and/or corrosion at 30-60 min, 24, 48, and 72 h after patch removal.

Based on the findings in this test the test substance is skin corrosing.