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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2010
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study
Qualifier:
according to guideline
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
Deviations:
no
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
yes
Vehicle:
no
Details on test solutions:
- direct weighing: the test item was added to 5 litres of dilution water
- then stirred for 24 hrs on a magnetic stirrer
- removing of undissolved particles of the test item by filtration using a folded filter of pore size 7-12 μm and an aseptic filter
- pH: 7.5 - 7.7
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
- mean standard body length (n=14): 3.5 cm (S.D. = 0.11 cm)
- Supplier: Aqua KlöGer (Germany) - loading: 0.62 g body weight (wet weight) per liter
- medication: none
- mortalities during acclimatisation period : < 5 %
- temperature: 20 - 24 °C
- feeding: commercial fish food (daily) and discontinued 24 h prior to test
- keeping water: synthetic fresh water in accordance with ISO 7346
Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Hardness:
13.9 °dH (= 248 mg/L CaCO3)
Test temperature:
22.4 to 23.3 °C (daily monitoring)
pH:
7.5 - 7.7
Dissolved oxygen:
7.5 - 8.7 mg/l
Salinity:
No data
Nominal and measured concentrations:
100 mg/L (nominal) plus control
Details on test conditions:
TEST SYSTEM:
- keeping water: synthetic fresh water according to ISO 7346
- photoperiod: 16 hours light, 8 hours dark
- temperature: 20 - 24 °C
- aeration: gentle aeration via narrow glass tubes
- no feeding during test
- test vessel: glass aquaria holding 5 L of test media covered with glass plates
- number of fish per test vessel: 7
- method of administration: direct weighing
- solubilizer or other auxiliaries: ultra-Turrax, magnetic stirrer, folded filter
- test concentration: 100 mg/l plus one control
- Method of initiation: animals were placed in prepared media
- test parameter: mortality (recorded daily)
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
Some test fish had a somewhat larger size than the one recommended in the test guideline for the zebra fish. This deviation is not regarded to be relevant to the results.
Validity criteria fulfilled:
yes
Remarks:
(mortality in the controls < 10% by the end of the test; dissolved oxygen concentration above 60% of the air-saturation value throughout the exposure period; pH did not vary by more than 1 unit.)
Conclusions:
In a semi-static test a LC50 of > 100 mg/l (n) after 96 h was obtained for the test item against fish (Danio rerio). No toxic effects against fish were observed at a limit test concentration of 100 mg/L under exposure conditions.
Executive summary:

The study was conducted under semi static conditions in accordance with Council Regulation (EC) No 440/2008, Method C.1 which is in most parts equivalent to the OECD Guideline for Testing of Chemicals No. 203. A group of seven fish of the recommended size was exposed to a limit test concentration of nominally 100 mg/L of the test item dissolved in dilution water. Auxiliaries used to prepare the test media were a magnetic stirrer, folded filters and an aseptic filter of pore size 0.2 μm. Observations were made on the number of dead fish and the incidence of sub-lethal effects after 2, 24, 48, 72 and 96 hours of exposure. No toxic effects against fish were observed at a limit test concentration of 100 mg/L under exposure conditions. The quantification of the test item was performed by DOC determination.

Description of key information

In a semi-static test  a LC50 of higher than 100 mg/l after 96 h was obtained for the test item against fish (Danio rerio). The result is expressed in terms of nominal concentrations. No toxic effects against fish were observed at a limit test concentration of 100 mg/L under exposure conditions. 

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
100 mg/L

Additional information

"Should read: > 100 mg/L"

Toxicity to fish was conducted in a study under semi static conditions in accordance with Council Regulation (EC) No 440/2008, Method C.1 which is in most parts equivalent to the OECD Guideline for Testing of Chemicals No. 203. A group of seven fish of the recommended size was exposed to a limit test concentration of nominally 100 mg/L of the test item dissolved in dilution water. Auxiliaries used to prepare the test media were a magnetic stirrer, folded filters and an aseptic filter of pore size 0.2 μm. Observations were made on the number of dead fish and the incidence of sub-lethal effects after 2, 24, 48, 72 and 96 hours of exposure. No toxic effects against fish were observed at a limit test concentration of 100 mg/L under exposure conditions. The quantification of the substance was performed by DOC determination.

The substance is insoluble or only poorly soluble in water. A suitable selective and sensitive chromatographical method for the determination of the test item in aqueous solutions could not be established. Therefore an accompanying analysis by means of a chromatographical method could not be performed. The quantification of the substance was performed by DOC determination.