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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Description of key information

After 72 hours no toxic effects against algae were observed at a limit test concentration of higher than 100 mg/L. The results are expressed in terms of nominal concentrations.

Key value for chemical safety assessment

EC50 for freshwater algae:
100 mg/L
EC10 or NOEC for freshwater algae:
100 mg/L

Additional information

"Should read: > 100 mg/L"

In 72 h the concentration of the test substance which reduces the growth rate of Scenedesmus subspicatus by 50 % is > 100 mg/l, relating to nominal concentration. The experiment was conducted in accordance with Commission Regulation (EC) No 761/2009 amending Regulation No 440/2008, Method C.3 which is equivalent to OECD Guideline for Testing of Chemicals No. 201 'Alga, Growth Inhibition Test' (2006).

Exponentially growing algal cells were exposed for a period of 72 hours to a limit test concentration of nominally 100 mg/L of the test item dissolved in dilution water. The cell densities were measured at 24 hour intervals. Inhibition of the algal population was measured as reduction in growth rate (index r), relative to control cultures grown under identical conditions. No toxic effects against algae were observed at a limit test concentration of 100 mg/L.

The test item is insoluble or only poorly soluble in water. A suitable selective and sensitive chromatographical method for the determination of the test item in aqueous solutions could not be established. Therefore an accompanying analysis by means of a chromatographical method could not be performed. The quantification of the test item was performed by DOC determination.