Registration Dossier

Diss Factsheets

Toxicological information

Acute Toxicity: oral

Currently viewing:

Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Study period:
7 Aug 1978 to 23 Aug 1978
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1978
Report date:
1978

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
GLP compliance:
not specified
Test type:
standard acute method

Test material

Constituent 1
Chemical structure
Reference substance name:
Isonicotinonitrile
EC Number:
202-856-2
EC Name:
Isonicotinonitrile
Cas Number:
100-48-1
Molecular formula:
C6H4N2
IUPAC Name:
isonicotinonitrile

Test animals

Species:
rat
Strain:
Wistar
Sex:
male
Details on test animals or test system and environmental conditions:
five groups of five male albino rats of Sherman-Wistar strain weighing between 200 and 300 grams. Deprived of food but not water for 24 hours prior to dosing.

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
water
Details on oral exposure:
Each animal weighed and dosed by direct administration of experimental material into the stomach by means of a syringe and dosing needle. Following administration, the animals were allowed food and water ad libitum for 14 day observation period, during which time rats were observed for signs of toxicity and mortality.
Doses:
250, 500, 1000, 2000, 4000 mg/kg
No. of animals per sex per dose:
5 males per dose
Control animals:
not specified

Results and discussion

Effect levels
Key result
Sex:
male
Dose descriptor:
LD50
Effect level:
710 mg/kg bw
Based on:
test mat.
95% CL:
>= 500 - <= 1 000
Mortality:
0/5 in 250 and 500 mg/kg groups.
1000 mg/kg group: deaths at day 3 (2), day 6 (2) and day 8 (1)
2000 mg/kg group: deaths at day 1 (4) and day 2 (1)
4000 mg/kg group: 5/5 dead at day 1
Clinical signs:
At 250 mg/kg, animals appeared slightly depressed after 2 hours but appeared normal after 24 hours. At 500 mg/kg, animals were lethargic after 1 hour and remained depressed for up to 48 hours. At 1000 mg/kg, animals were depressed after 30 minutes and exhibited shallow breathing. Condition deteriorated over next 48 hours with deaths occurring over next several days. At 2000 mg/kg and 4000 mg/kg, animals were comatose within 1 hour and generally died within 2 hours.
Body weight:
250 mg/kg: Initial weight: 255 grams; final weight 280 grams
500 mg/kg: Initial weight: 260 grams; final weight 280 grams
Gross pathology:
Gross pathologic examination revealed nothing remarkable.

Applicant's summary and conclusion

Interpretation of results:
Category 4 based on GHS criteria
Conclusions:
Acute oral LD50 (rat) for 4-Cyanopyridine is 710 mg/kg.
Executive summary:

Acute oral LD50 (rat) for 4-Cyanopyridine is 710 mg/kg.