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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
2013

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 471 (Bacterial Reverse Mutation Assay)
GLP compliance:
yes
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
Isonicotinonitrile
EC Number:
202-856-2
EC Name:
Isonicotinonitrile
Cas Number:
100-48-1
Molecular formula:
C6H4N2
IUPAC Name:
isonicotinonitrile
Specific details on test material used for the study:
99.65% purity

Method

Species / strain
Species / strain / cell type:
S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2
Metabolic activation:
with and without
Metabolic activation system:
rat liver, induced with phenobarbital and 5,6-benzoflavone
Test concentrations with justification for top dose:
without S9: 0, 2.29, 6386, 20.6, 61.7, 185, 556, 1667, 5000 ug/plate (all strains: dose determination test). Main test: 0, 313, 625, 1250, 2500, 5000 ug/plate (all strains)
with S9: 0, 2.29, 6386, 20.6, 61.7, 185, 556, 1667, 5000 ug/plate (all strains: dose determination test). Main test: 0, 313, 625, 1250, 2500, 5000 ug/plate (all strains)
Vehicle / solvent:
DMSO
Controls
Untreated negative controls:
yes
Negative solvent / vehicle controls:
yes
True negative controls:
no
Positive controls:
yes
Positive control substance:
9-aminoacridine
sodium azide
other: 2-(2-Furyl)-3-(5-nitro-2-furyl)acrylamide (TA100, WP2, TA98)
Details on test system and experimental conditions:
Procedure: pre-incubation method, S9 concentration: 10% for dose determination test, 10% and 30% for main test, 3 plates/test, 2 replicates

Results and discussion

Test resultsopen allclose all
Key result
Species / strain:
S. typhimurium TA 1535
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Untreated negative controls validity:
valid
Positive controls validity:
valid
Key result
Species / strain:
S. typhimurium TA 1537
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Untreated negative controls validity:
valid
Positive controls validity:
valid
Key result
Species / strain:
S. typhimurium TA 98
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Untreated negative controls validity:
valid
Positive controls validity:
valid
Key result
Species / strain:
S. typhimurium TA 100
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Untreated negative controls validity:
valid
Positive controls validity:
valid
Key result
Species / strain:
E. coli WP2 uvr A pKM 101
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Untreated negative controls validity:
valid
Positive controls validity:
valid

Applicant's summary and conclusion

Conclusions:
Pyridine-4-carbonitrile was assessed to be non-mutagenic (negative) in the reverse mutation test in bacteria under the test conditions. No growth inhibition of the bacterial lawn with the chemical was observed in all tester strains with or without metabolic activation.
Executive summary:

Pyridine-4-carbonitrile was assessed to be non-mutagenic (negative) in the reverse mutation test in bacteria under the test conditions.  No growth inhibition of the bacterial lawn with the chemical was observed in all tester strains with or without metabolic activation.