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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Justification for type of information:
REPORTING FORMAT FOR THE ANALOGUE APPROACH

1. HYPOTHESIS FOR THE ANALOGUE APPROACH
This read-across is based on the hypothesis that source and target substances have similar toxicological properties because
• they are manufactured from similar or identical precursors under similar conditions
• they share structural similarities with common functional groups: methacrylate esters
• the metabolism pathway leads to comparable products (methacrylic acid and short chain alcohol).

Therefore, read-across from the existing (eco)toxicity studies on the source substances is considered as an appropriate adaptation to the standard information requirements of REACH regulation

2. SOURCE AND TARGET CHEMICAL(S) (INCLUDING INFORMATION ON PURITY AND IMPURITIES)
see cross reference “Justification for read-across”

3. ANALOGUE APPROACH JUSTIFICATION
see cross reference “Justification for read-across”

4. DATA MATRIX
see cross reference “Justification for read-across”
Reason / purpose for cross-reference:
read-across source
Reason / purpose for cross-reference:
read-across: supporting information
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Test type:
semi-static
Water media type:
freshwater
Total exposure duration:
96 h
Reference substance (positive control):
no
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
70.7 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality (fish)
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
52.5 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality (fish)
Duration:
72 h
Dose descriptor:
LC50
Effect conc.:
50.4 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
48.7 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality (fish)
Validity criteria fulfilled:
yes
Conclusions:
The 96 h LC50 in Danio rerio (zebrafish) was 48.7 mg a.i./L.

Description of key information

96 h LC50 = 48.7 mg/L; OECD guideline 203; GLP; read-across from ETMA

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
48.7 mg/L

Additional information

No acute toxicity data in freshwater fish are available for MTMA. However, reliable (RL1-2) data are available for ETMA. A justification for read-across is given in the endpoint summary "Aquatic toxicity".

In a 96-h acute toxicity study according to OECD Guideline 203, adopted 17 July 1992, Danio rerio (zebrafish) were exposed to ETMA at nominal concentrations of 0 (control), 6.25, 12.5, 25, 50 and 100 mg a.i./L under static renewal conditions.

The 96 h LC50 was 48.7 mg/L (based on nominal concentrations).

Sublethal effects like lethargy, slowed or no escape reflex and hyperventilation were observed in the 50 mg/L group; no abnormalities were observed up to a concentration of 25 mg/L.

LC50 values of 62.3 mg/L for MTMA and 30.97 mg/L for ETMA were predicted using ECOSAR V1.11. This supports the hypothesis, that effect levels in the same order of magnitude can be expected for the target and source substance. Thus, the result obtained with the source substance ETMA can be used as surrogate date for the target substance MTMA.