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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vitro / ex vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Guideline study to GLP
Confirms irritancy of simple organic acid salts of chromium III
Acetate salt is seen as a suitable surrgoate for propionate; the two acids are similar and are both water soluble under the conditions of testing and the slightly larger molecular weight propionic is less hazardous than the acetic acid

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2017
Report date:
2017

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 492 (Reconstructed Human Cornea-like Epithelium (RhCE) Test Method for Identifying Chemicals Not Requiring Classification and Labelling for Eye Irritation or Serious Eye Damage)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Chromium acetate
EC Number:
241-562-9
EC Name:
Chromium acetate
Cas Number:
17593-70-3
Molecular formula:
3Cr, < 3(C2H4O2) < 1(OH)
IUPAC Name:
Chromium III hydroxyacetate

Results and discussion

In vitro

Results
Irritation parameter:
in vitro irritation score
Run / experiment:
1
Value:
ca. 4.4
Vehicle controls validity:
valid
Negative controls validity:
valid
Positive controls validity:
valid
Other effects / acceptance of results:
The low viability is close to figures seen for Eye Dam 1.

Applicant's summary and conclusion

Interpretation of results:
Category 2 (irritating to eyes) based on GHS criteria
Conclusions:
It is noted that this method alone cannot validate the division between Eye Dam 1 and Eye Irrit 2, but acetates are weak acids and although the class if substance will show significant effect in this in-vitro assay, impact on the cornea (ie Eye Dam 1) is not expected.
Citric acid is a weaker acid than acetic and this result suggests that Eye Irrit 2 is a valid classification.