Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 203-694-5 | CAS number: 109-67-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 01 June 2017 to 31 July October 2017
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 310 (Ready Biodegradability - CO2 in Sealed Vessels (Headspace Test)
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- EAG test substance NO. 13777
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Duration of test (contact time):
- 21 d
- Initial conc.:
- 17 mg/L
- Based on:
- other: C
- Parameter followed for biodegradation estimation:
- inorg. C analysis
- Details on study design:
- 1. Test vessels: 160 mL glass serum bottles filled with 107 mL test medium containing 4 mg/L inoculum.
2. Test substance dosing: Preliminary work showed that pentene-1 is miscible with silicone oil AR20 and not from the solution in a closed container. For ready biodegradability test, pentene-1 was mixed with silicone oil AR 20 (2) as the dosing stock solution. Specifically, 1.8303 g silicone oil was mixed with 60 µL pentene 1 in a 2.0 mL HPLC glass vial and the vial was immediately capped. Then 100 µL of the mixed stock was dosed to each serum bottle to give a final concentration of ~17 mg C/L. The sample bottles were immediately crimp-sealed and the solution was rigorously mixed by hand shaking.
3. Reference dosing: A 3.5 µL aliquot of pure 1-octanol was directly added to each serum bottle to give a final concentration of ~20 mg C/L. The bottles were immediately crimp-sealed and the solution was rigorously mixed by hand shaking.
4. Control: A 100 µL aliquot of pure silicone oil AR20 was directly added to each serum bottle to serve as the background control. The bottles were immediately crimp-sealed and the solution was rigorously mixed by hand shaking.
5. Incubation: The sample bottles were laid horizontally and placed on a platform shaker with constant oscillation at ~ 120 rpm.
6. Sampling: On Days 7, 15, and 21, sample bottles were injected with 1 mL of 7 N NaOH, respectively and were further processed for IC analysis. - Reference substance:
- other: 1-octanol
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0.6
- Sampling time:
- 21 d
- Details on results:
- No biodegradation was observed for pentene-1 under the test conditions. This is likely due to that petene-1 was not bioavailable for microbes presented in activated sludge inoculum. Pentene-1 is extremely volatile and water insoluble, it could migrate to the headspace of the sample bottles even under constant agitation of the test medium.
- Results with reference substance:
- For reference material, more than 70% of 1-octanol was degraded in 15 days, indicating that the test system was biologically viable.
- Validity criteria fulfilled:
- not applicable
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- Under the test conditions in accordance with OECD 310 guideline and using silicone oil AR 20 to facilitate dispersion of the test material, pentene-1 still was not biodegraded in test medium containing activated sludge inoculum.
- Executive summary:
The feasibility of testing pentene-1 in sealed vessels (headspace test) following OECD 310 study guideline was studied in a preliminary work. For reference material, more than 70% of 1-octanol was degraded in 15 days, indicating that the test system was biologically viable. No biodegradation was observed for pentene-1 under the test conditions. This is likely due to that pentene-1 was not bioavailable for microbes presented in activated sludge inoculum. Pentene-1 is extremely volatile, it could migrate to the headspace of the sample bottles even under constant agitation of the test medium.
Under the test conditions in accordance with OECD 310 guideline and using silicone oil AR 20 to facilitate dispersion of the test material, pentene-1 still was not biodegraded in test medium containing activated sludge inoculum.
As no biodegradation of pent-1 -ene was observed in this preliminary work, no further study was performed. This study is therefore considered valid with restrictions despite the fact that it was not carried out under GLP after REACH came into force. The substance is considered as not readily biodegradable.
Reference
Description of key information
Study similar to OECD 310, non-GLP, key study, validity 2:
0.6% of biodegradation after 21 days (inoculum: activated sludge)
Under test conditions, the percentage biodegradation of the test item did not reach 60% in 10 day-window, so the substance can not be considered as readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
One valid key study is available (EAG, 2017) to assess the readily biodegradation of the registered substance as a preliminary test.This study was performed to determine any potential for biodegradability of the test item using a method designed for highly volatile substances: the sealed vessels (headspace test) following OECD 310 study guideline.
For reference material, more than 70% of 1-octanol was degraded in 15 days, indicating that the test system was biologically viable. No biodegradation was observed for pentene-1 under the test conditions. This is likely due to that pentene-1 was not bioavailable for microbes presented in activated sludge inoculum. Pentene-1 is extremely volatile, it could migrate to the headspace of the sample bottles even under constant agitation of the test medium.
Under the test conditions in accordance with OECD 310 guideline and using silicone oil AR 20 to facilitate dispersion of the test material, pentene-1 still was not biodegraded in test medium containing activated sludge inoculum.
As no biodegradation of pent-1 -ene was observed throughout this preliminary work, it was not considered useful to perform a definitive study under GLP as no degradation would be expected. This study is therefore considered valid with restrictions despite the fact that it was not carried out under GLP after REACH came into force. The substance is considered as not readily biodegradable.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
