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EC number: 203-694-5 | CAS number: 109-67-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods with acceptable restrictions
- Remarks:
- Well documented study but not performed under GLP. Testguideline similar to OECD test guideline, but the organism E.Coli was not tested. Substance purity not reported.
Data source
Reference
- Reference Type:
- other: Study result available in the National Toxicology Program database
- Title:
- Unnamed
- Year:
- 2 002
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- other: NTP Standard Protocol
- Deviations:
- yes
- Remarks:
- The test was not conducted with E. Coli
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- The test was not conducted with E. Coli
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Pent-1-ene
- EC Number:
- 203-694-5
- EC Name:
- Pent-1-ene
- Cas Number:
- 109-67-1
- Molecular formula:
- C5H10
- IUPAC Name:
- pent-1-ene
- Test material form:
- not specified
Constituent 1
Method
- Target gene:
- histidine gene
Species / strain
- Species / strain / cell type:
- bacteria, other: S. Typhimurium TA97, TA98, TA100, TA1535
- Metabolic activation:
- with and without
- Metabolic activation system:
- metabolic activation enzymes and cofactors from Aroclor 1254-induced male Sprague-Dawley rat or Syrian hamster liver S9 (10 and 30%)
- Test concentrations with justification for top dose:
- 0, 100, 333, 1000, 3333, 10000 µg/plate
- Vehicle / solvent:
- Dimethyl Sulfoxide (DMSO)
Controlsopen allclose all
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- 2-nitrofluorene
- sodium azide
- Remarks:
- without S9
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene (or occasionnally, sterigmatocystin)
- Remarks:
- with S9
- Details on test system and experimental conditions:
- METHOD OF APPLICATION:
preincubation
DURATION
- Preincubation period: 20 minutes
- Exposure duration: 2 days
Results and discussion
Test results
- Species / strain:
- S. typhimurium, other: S. typhimurium TA 1535, TA 97, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Remarks:
- only slight cytotoxicity was measured for 10000 ug/plate without HLI
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
Any other information on results incl. tables
See attachment in section "Attached background material".
Applicant's summary and conclusion
- Conclusions:
- Under the test condition,1-Pentene is not mutagenic with and without metabolic activation in S. typhimurium strains (TA1535, TA97, TA98 and TA100) according to the criteria of the Annex I of the of the Regulation (EC) No. 1272/2008 (CLP) and to the GHS.
- Executive summary:
In a reverse gene mutation assay performed according to the NTP Standard Protocol (Ames Test), Salmonella typhimurium strains TA 1535, TA 97 and TA 98 and TA 100 were exposed to 1-pentene diluted in DMSO both in the presence and absence of metabolic activation system (S9 fraction) from 100 to 10000 µg/plate using the preincubation method. Vehicle (dimethyl sulphoxide) and positive control groups were also included in mutagenicity tests.
No test material precipitate was observed on the plates at any of the doses tested in either the presence or absence of S9-mix.
All of the positive control chemicals used in the test induced marked increases in the frequency of revertant colonies, both with or without metabolic activation. Thus, the sensitivity of the assay and the efficacy of the S9-mix were validated.
No significant increases in the frequency of revertant colonies were recorded for any of the bacterial strains, with any dose of the test material, either with or without metabolic activation.
Under the test condition, test material is not mutagenic with and without metabolic activation in S. typhimurium (strains TA1535, TA97, TA98 and TA100) according to the criteria of the Annex I of the of the Regulation (EC) No. 1272/2008 (CLP) and to the GHS.
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