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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Justification for type of information:
Please refer to the attached analogue justification provided in IUCLID Section 13.
Reason / purpose for cross-reference:
read-across source
Reason / purpose for cross-reference:
read-across source
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
58.4 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: Source: 6.1.3-1
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
56 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: Source: Van Leeuwen, 1985
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
9 May - 16 June 2000
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes
Remarks:
according to the GLP standard by the Japanese Ministry of Environment
Specific details on test material used for the study:
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: at room temperature in the dark


Analytical monitoring:
yes
Details on sampling:
- Concentrations: all test item concentrations were analyzed
Vehicle:
no
Details on test solutions:
According to guideline
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Age of parental stock: between 0 - 24 h
- Feeding during test: no feeding during test

ACCLIMATION
- Acclimation period: 14 d
- Type and amount of food: Chlorella vulugaris, 0.1 - 0.2 mgC/d


Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Test temperature:
20.4 - 20.7 °C (original test)
20.0 - 20.4 °C (supplemental test)
pH:
7.0 - 7.4 (control in the original test)
7.1 - 8.8 (test substance in the original test)

6.9 - 7.0 (control in the supplemental test)
6.9 - 10.7 (test substance in supplemental test)
Dissolved oxygen:
8.3 - 8.6 mg/L (control in the original test)
8.4 - 8.6 mg/L (test substance in the original test)

7.9 - 8.1 mg/L (control in the supplemental test)
8.2 - 8.9 mg/L (test substance in supplemental test)
Nominal and measured concentrations:
Nominal concentration in the original test
0.79, 1.43, 2.57, 4.63, 8.33, and 15.0 mg/L
Geometric mean of measured concentration in the original test
0.57, 1.07, 1.89, 3.52, 6.41, and 12.6 mg/L

Nominal concentration in the supplemental test
27.0, 48.6, 87.5, and 157.5 mg/L
Geometric mean of measured concentration in the supplemental test
24.9, 45.3, 81.9, and 149.0 mg/L
Details on test conditions:
TEST SYSTEM
- Fill volume: 100 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline
- Culture medium different from test medium: no
- Intervals of water quality measurement: the content of the test item, pH and concentration of dissolved oxygen were measured at the beginning and at the end of the test

OTHER TEST CONDITIONS
- Photoperiod: light/dark 16/8 h

EFFECT PARAMETERS MEASURED: after 24 and 48 h the immobilised Daphnids were counted

TEST CONCENTRATIONS
- Range finding study: yes, without analytics, not further data in the report
Reference substance (positive control):
yes
Remarks:
Potassium dichromate
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
58.4 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
24.9 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
- Mortality of control: no mortality observed
Results with reference substance (positive control):
- Results with reference substance valid? Yes
- EC50: 0.60 mg/L

Table 1. Measured concentrations of test substance

Original test

Nominal concentration (mg/L)

Measured concentration (mg/L)

Percent of nominal (%)

0 hour

new

48 hour

old

Geometric mean

0 hour

new

48 hour

old

Control

< 0.04

< 0.04

-

-

-

0.79

0.58

0.56

0.57

73.4

70.9

1.43

1.08

1.06

1.07

75.5

74.1

2.57

1.90

1.88

1.89

73.9

73.2

4.63

3.39

3.65

3.52

73.2

78.8

8.33

6.37

6.45

6.41

76.5

77.4

15.0

12.7

12.6

12.6

84.7

84.0

 

Supplemental test

Nominal concentration (mg/L)

Measured concentration (mg/L)

Percent of nominal (%)

0 hour

new

48 hour

old

Geometric mean

0 hour

new

48 hour

old

Control

< 0.04

< 0.04

-

-

-

27.0

25.4

24.4

24.9

94.1

90.4

48.6

45.9

44.8

45.3

94.4

92.2

87.5

83.3

80.5

81.9

95.2

92.0

157.5

151.1

146.9

149.0

95.9

93.3

 

New: freshly prepared test solutions

Old: test solutions after 48 hours exposure period

 

Table 2. Immobility of Daphnia magna exposed to test substance

Original test

Measured concentration (mg/L)

Cumulative number of dead or immobilized daphnia

(percent mortality or immobility)

24 hour

48 hour

Control

0 (0)

0 (0)

0.57

0 (0)

0 (0)

1.07

0 (0)

0 (0)

1.89

0 (0)

0 (0)

3.52

0 (0)

0 (0)

6.41

0 (0)

0 (0)

12.6

0 (0)

0 (0)

 

Supplemental test

Measured concentration (mg/L)

Cumulative number of dead or immobilized daphnia

(percent mortality or immobility)

24 hour

48 hour

Control

0 (0)

0 (0)

24.9

0 (0)

0 (0)

45.3

1 (5)

10 (50)

81.9

8 (40)

12 (60)

149.0

20 (100)

20 (100)

Conclusions:
The LC50 of the test substance ammounts 56 mg/L when tested under the conditions of this assay (OECD TG 202).
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1985
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
not specified
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Water flea

ACCLIMATION
- Type and amount of food: C. pyrenoidosa (1 x 10E8 cells/l)
Limit test:
no
Total exposure duration:
48 h
Details on test conditions:
TEST SYSTEM
- Test vessel: sealed vessels
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
56 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% CI: 32 - 100 mg/L
Reported statistics and error estimates:
LC50 values an their 95% confidence intervals were calculated according to the Litchfield and Wilcoxon (1949) method
Validity criteria fulfilled:
not specified
Conclusions:
The LC50 of diethylamine ammounts to 56 mg/L when tested under the conditions of this assay (OECD TG 202).

Description of key information

EC50 (48 h) = 56 mg/L (OECD 202) based on read across from CAS 109-89-7

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
56 mg/L

Additional information

Data on the toxicity of Diethylammonium chloride (CAS 660-68-4) to aquatic invertebrates are not available. Therefore read across to the analogues substance N-ethylethanamine (CAS 109-89-7) was conducted.

A reliable study (reference: 6.1.3-1) is available testing the toxicity of N-ethylethanamine (CAS 109-89-7) to aquatic invertebrates according to OECD 202 and GLP standards. The study was conducted in two steps. In the first part Daphnia magna, as the test organism was exposed for 48 h to test item concentrations of 0.79, 1.43, 2.57, 4.63, 8.33, and 15.0 mg/L (nominal). In a second step higher concentrations were tested (27.0, 48.6, 87.5, and 157.5 mg/L, nominal). Both tests were performed under static test conditions. An analytical monitoring was performed via HPLC. The recoveries of the substance were between 70 and 85% of the nominal values in the first test and > 90% in the second test. Therefore the results are expressed as geometric mean measured concentrations. The study resulted in an EC50 (48 h) of 58.4 mg/L (geom. mean meas.).

This result is in agreement with the findings of Van Leeuwen et al. (1985). The toxicity of N-ethylethanamine (CAS 109-89-7) to Daphnia magna was tested according to the guideline OECD 202. The study reported an EC50 (48 h) value of 56 mg/L (nominal). Since the publication is poorly documented it was used as the supporting study.

Similar effect levels to aquatic invertebrates are expected from the target substance Diethylammonium chloride (CAS 660-68-4).