Diethylammonium chloride

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

activated sludge, domestic

Details on inoculum:

- Inoculum: activated sludge from domestic wwtp
- Volume: 200 ml (50 ml activated sludge (1 g/l dry matter), 8 ml of synthetic medium
- Preparation of inoculum for exposure: washed activated sludge from a continuously operating laboratory activated sludge unit treating domestic sewage was used

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

30 min

Test temperature:

room temperature: 20-25°C

Details on test conditions:

TEST SYSTEM
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Duration:

30 min

Dose descriptor:

EC10

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Results with reference substance (positive control):

- Relevant effect levels: EC50 (30 min) 4.5 mg/L (nominal)

No respiration inhibition up to 1000 mg/l, the highest concentration tested

Conc.              Respiration
                       [mgO2/l*h]                  % of blank
Blank              17                               -
100 mg/l          19                               112
1000 mg/l        19                               112

A disturbance of sewage works in WWTP is not expected (EC20 > 100 mg/l).

Reference 2

Endpoint:

toxicity to microorganisms

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

Please refer to the attached analogue justification provided in IUCLID Section 13.

Reason / purpose for cross-reference:

read-across source

Duration:

30 min

Dose descriptor:

EC10

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Description of key information

EC10 (30 min) > 1000 mg/L (nominal, ISO 8192) based on read across from CAS 109-89-7

Additional information

Experimental data on the toxicity of Diethylammonium chloride (CAS 660-68-4) towards microorganisms are not available. Therefore appropriate read across data from the analogue substance N-ethylethanamine (CAS 109-89-7) were used for the environmental assessment.

The available study examines the toxicity of the read across substance N-ethylethanamine (CAS 109-89-7) to activated sludge microorganisms and was performed according to ISO guidance 8192. Activated sludge from a domestic sewage treatment plant was exposed to nominal concentrations of 100 and 1000 mg test item/L. After an exposure time of 30 min no inhibition of the respiration was recorded, resulting into an EC10 of > 1000 mg/L.

Furthermore N-ethylethanamine (CAS 109-89-7) was found to be readily biodegradable (OECD 301 C), which provides additional evidence, that the substance is not harmful to microorganisms.

Based on the results of the analogue substance, Diethylammonium chloride (CAS 660-68-4) is not considered to be toxic to microorganisms.