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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
24.10.2017 to 26.10.2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2017

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
2004
GLP compliance:
yes (incl. QA statement)
Remarks:
BioChem agrar, Labor für biologische und chemische Analytik GmbH, Kupferstr. 6, 04827 Machem OT Gerichshain (Germany)

Test material

Constituent 1
Chemical structure
Reference substance name:
3,7-dimethyloctane-1,7-diol
EC Number:
203-517-1
EC Name:
3,7-dimethyloctane-1,7-diol
Cas Number:
107-74-4
Molecular formula:
C10H22O2
IUPAC Name:
3,7-dimethyloctane-1,7-diol
Test material form:
liquid
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Miltitz Aromatics GmbH (Bitterfeld-Wolfen, Germany), Batch No.: BM 7/17, Lot No.: 239/17
- Expiration date of the lot/batch: 14.06.2019
- Purity test date: 14.06.2017

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: hydroxycitronellol was stored in the original container, at room temperature (at BioChem agrar GmbH: at ca. 20°C, dark and dry)
- Stability under test conditions: yes

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: no
- Final dilution of a dissolved solid, stock liquid or gel: Due the water solubility of the test item ca. 30 g/L at 25°C), a stock solution was prepared. The desired test concentration of ca. 100 mg/L was achieved by dilution.

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
The test item hydroxycitronellol is soluble in water (ca. 30 g/L at 25°C). A stock solution was prepared by weighing of 53 mg test item into a graduated flask and filling up to 500 mL using test medium. The test solutions were prepared directly in the test vessels.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea (Daphnia magna Straus)
- Source: Origin of the test organism: RWTH Aachen University, Institute for Environmental Research (Biology V), Worringerweg 1, 52074 Aachen Germany); origin of the animals used in the test: test animals held and bred in the test facility under standardised laboratory conditions, test animals derived from a healthy stock, not first brood progeny
- Age of parental stock (mean and range, SD): not reported
- Feeding during test: no

ACCLIMATION: not necessary

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Hardness:
232 mg/L CaCO3 (test medium)
Test temperature:
19.7-20.5°C in all test vessels during the test
pH:
7.76-7.87 in all test vessels during the test
Dissolved oxygen:
8.67-8.79 mg/L in all test vessels during the test
Conductivity:
1.3 µS/cm (test medium)
Nominal and measured concentrations:
100 mg/L nominal
Details on test conditions:
TEST SYSTEM
- Test vessel: glass beaker
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass/25 mL/-/10 mL
- Aeration: no
- Type of flow-through (e.g. peristaltic or proportional diluter): study conducted under static conditions
- Renewal rate of test solution (frequency/flow rate): not applicable (study conducted under static conditions)
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4 (+3 additional vessels: 1 for measuring of water parameters, 1 for analysis, 1 retain specimen)
- No. of vessels per control (replicates):4 (+3 additional vessels: 1 for measuring of water parameters, 1 for analysis, 1 retain specimen)

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline
- Alkalinity: 0.8 mmol/L
- Ca/Mg ratio: 4:1
- Conductivity: 1.3 µS/cm
- Culture medium different from test medium: no
- Intervals of water quality measurement:

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light/8 h dark
- Light intensity: ca. 20 µE/m² x s

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mobility

VEHICLE CONTROL PERFORMED: no (no veheicle used)

RANGE-FINDING STUDY: performance not reported
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
LOEC
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Results with reference substance (positive control):
In the most recent study, the 24 h EC50 was determined to be 1.34 mg/L potassium dichromate. Thus, the reference item EC50 was within the expected range of toxicity (1.0-2.0 mg/L).
Reported statistics and error estimates:
Due to the test results, nstatistical calculations were performed.o

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
In an acute toxicity test, the 48 h NOEC, LOEC, and EC50 values based on a nominal test concentration were determined to be >=100, >100, and >100 mg/L hydroxycitronellol, respectively.
Executive summary:

The acute toxicity of hydroxycitronellol to Daphnia magna was investigated in a study conducted according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test; 2004). In this limit test, 20 daphnids were exposed to a nominal concentration of 100 mg/L under static conditions for 48 h. Immobility and abnormal behaviour/symptoms of the test animals were recorded. The 48 h NOEC, LOEC, and EC50 values based on a nominal test concentration were determined to be >=100, >100, and >100 mg/L, respectively.

The study is well documented and can be classified as reliable without restrictions satisfying the guideline requirements for an acute toxicity study with invertebrates.

Results Synopsis

Test organism/age: Daphnia magna (<24 h old)

Test Type: static

48 h NOEC: >=100 mg/L (nominal)

48 h LOEC: >100 mg/L (nominal)

48 h EC50: >100 mg/L (nominal)

Endpoint(s) Effected: mobility, behaviour/symptoms