Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 300-491-4 | CAS number: 93940-93-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017-08-08 to 2017-10-18
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 2004
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Bis[N-(2-hydroxyethyl)-N-methylglycinato-N,O,ON]copper
- EC Number:
- 300-491-4
- EC Name:
- Bis[N-(2-hydroxyethyl)-N-methylglycinato-N,O,ON]copper
- Cas Number:
- 93940-93-3
- Molecular formula:
- C10H20CuN2O6
- IUPAC Name:
- bis[N-(2-hydroxyethyl)-N-methylglycinato-N,O,ON]copper
- Test material form:
- solid: particulate/powder
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: analytical samples taken from control and all test item concentrations at 0 hours (initial value) and 48 hours aged test solutions were analysed
- Fortified samples: a stock solution (1770 mg/L, purity not considered) and a dilution (1 mg/L) were prepared in acetonitrile/water (1:1, v/v) +0.1% acetic acid and used for fortification of recovery samples.
- Sample storage (before test): stored deep-frozen until analysis
Test solutions
- Vehicle:
- no
- Details on test solutions:
- - Stock solution of 15 mg/L. Lower test concentrations were prepared by dilution.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: straus, clone 5
- Feeding during test: no feeding during test
- Feeding of culture (before test): three times a week with single cell green algae
- Main test: freshly hatched daphnids less than 24 hours old were used
Study design
- Test type:
- static
- Water media type:
- freshwater
- Remarks:
- Elendt M4 medium
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 250 mg/L CaCO3
- Test temperature:
- 20.2 – 21.7 °C
- pH:
- 7.28 – 8.03
- Dissolved oxygen:
- ≥ 8.3 mg/L
- Nominal and measured concentrations:
- Nominal concentrations: 1.50, 0.500, 0.167, 0.0556 and 0.0185 mg/L and a control
Measured concentration (geometric mean based): 1.70, 0.515, 0.170, 0.0556, 0.0189 mg/L and not determined for the control - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass vessel
- Type: covered with a glass plate (to reduce evaporation)
- Material, size, headspace, fill volume: 100 mL capacity filled with 50mL of test solution
- No. of organisms: 20 daphnids per treatment group (4 replicates of 5 animals each)
- No. of treatment groups: 6 (5 dose rates of the test item and a control)
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4
- Culture medium different from test medium: same as Elendt M4
OTHER TEST CONDITIONS
- Exposure to light: none
EFFECT PARAMETERS MEASURED: Immobilisation
RANGE-FINDING STUDY
- Test concentrations: control, 100, 10 and 1 mg/L
- Sampling points : after 24h and 48h of exposure
- Results used to determine the conditions for the definitive study:
-- After 24 h 95 % immobilisation was observed at 10.0 mg/L. 100 % of the daphnids were found to be immobile at the highest test item concentration of 100 mg/L.
-- After 48 hours 100 % immobilisation was observed at 10.0 mg/L and 100 mg/L test item. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate, tested at 1.00 and 2.00 mg/L with 20 test organisms per test concentration, at around the same time period as the study
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.114 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.056 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- see any other information on results
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Test concencentrations: 1 and 2 mg/L (20 organisms per test concentration)
- Relevant effect levels: EC50 (24h) is between 1 and 2 mg/L. The results are in accordance with the requirements of the OECD guideline 202.
Any other information on results incl. tables
Results non-GLP range finding test
After 48h
nominal test concentration (mg/L) | ||||
control | 1 | 10 | 100 | |
group 1 | 0 | 0 | 5 | 5 |
group 2 | 0 | 0 | 5 | 5 |
group 3 | 0 | 1 | 5 | 5 |
group 4 | 0 | 0 | 5 | 5 |
sum | 0 | 1 | 20 | 20 |
% | 0 | 5 | 100 | 100 |
Summary of biological results:
a) Results of the test, 24h values
|
nominal test item concentration [mg/L] |
|||||
|
Control |
0.0185 |
0.0556 |
0.167 |
0.500 |
1.50 |
immobilised daphnids after 24 h |
||||||
Group 1 |
0 |
0 |
0 |
2 |
2 |
5 |
Group 2 |
0 |
0 |
1 |
1 |
3 |
5 |
Group 3 |
0 |
0 |
0 |
2 |
2 |
5 |
Group 4 |
0 |
0 |
1 |
2 |
5 |
5 |
Σ |
0 |
0 |
2 |
7 |
12 |
20 |
% |
0 |
0 |
10 |
35 |
60 |
100 |
b) Results of the test, 48h values
|
nominal test item concentration [mg/L] |
|||||
|
Control |
0.0185 |
0.0556 |
0.167 |
0.500 |
1.50 |
immobilised daphnids after 48 h |
||||||
Group 1 |
0 |
0 |
0 |
4 |
5 |
5 |
Group 2 |
0 |
0 |
1 |
5 |
5 |
5 |
Group 3 |
0 |
0 |
0 |
4 |
5 |
5 |
Group 4 |
0 |
0 |
1 |
4 |
5 |
5 |
Σ |
0 |
0 |
2 |
17 |
20 |
20 |
% |
0 |
0 |
10 |
85 |
100 |
100 |
Determined concentration of test substance:
Test item |
Sampling |
Test item found |
Test item concentration measured |
|
nominal [mg/L] |
[h] |
[mg/L] |
% of nominal |
[mg/L] |
Control |
0 fresh |
< LOD |
- |
- |
48aged |
< LOD |
- |
- |
|
0.0185 |
0 fresh |
0.0187 |
101 |
0.0189 |
48 aged |
0.0192 |
104 |
||
0.0556 |
0 fresh |
0.0558 |
100 |
0.0556 |
48 aged |
0.0564 |
101 |
||
0.167 |
0 fresh |
0.175 |
105 |
0.170 |
48 aged |
0.166 |
99 |
||
0.500 |
0 fresh |
0.528 |
106 |
0.515 |
48 aged |
0.498 |
100 |
||
1.50 |
0 fresh |
1.60 |
107 |
1.70 |
48 aged |
1.79 |
119 |
Effect concentrations:
nominal concentrations | measured concentrations | |||
24h | 48h | 24h | 48h | |
NOEC | 0.0556 | 0.0556 | 0.0556 | 0.0556 |
EC50 | 0.269 | 0.102 | 0.335 | 0.114 |
95% confidence limit of EC50 | 0.186-0395 | 0.079-0.132 | 0.225-0.475 | 0.090-0.139 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48-hour EC50 for Daphnia magna is 0.114 mg/L (measured, geom. mean).
- Executive summary:
The short-term toxicity to Daphnia magna was determined according to OECD 202 guideline with no deviations. The study was conducted under static conditions. The daphnids were exposed over a period of 48 hours to a nominal concentrations of 1.50, 0.500, 0.167, 0.0556 and 0.0185 mg/L and a control. This corresponds to 1.70, 0.515, 0.170, 0.0556, 0.0189 mg/L (geometric mean of measured concentrations).
The validity criteria were fulfilled.
The EC50 value was determined to be 0.114 mg/L (measured, geom. mean) and the NOEC 0.0556 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
