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EC number: 220-239-6 | CAS number: 2682-20-4
Table 1. Median lethal concentrations (LC5Os) from the toxicity test with 2-methyl-4- isothiazolin-3-one, Technical and the rainbow trout, Oncorhynchus mykiss.
The acute toxicity of 2-methyl-4-isothiazolin-3-one, Technical to the rainbow trout, Oncorhynchus mykiss, is described in this report). The test substance, 2-methyl-4-isothiazolin-3-one, Technical, was supplied by the sponsor and is synonymous with RH-573 and RH-57,399 Technical. The sample was identified as Lot No. B- 1001, TD No. 01-043, with a % a.i. of 94.3%.
The test was performed under flow-through conditions with five concentrations of test substance and a dilution water control at a mean temperature of 11.9°C. The dilution water was filtered deionized water collected at Marblehead, Massachusetts and adjusted to a hardness of 40 to 48 mg/L as CaCO3- No cloudiness or insoluble material was observed in any test vessel during the definitive test. All water quality parameters (dissolved oxygen concentration, temperature, pH, and conductivity) were within acceptable limits.
Based on the initial diluter calibration, nominal concentrations of 2-methyl-4-isothiazolin-3-one, Technical were: 0 mg/L (control), 1.3, 2.2, 3.6, 6.0, and 10 mg a.i./L. Mean measured concentrations of 2-methyl-4-isothiazolin-3- one were: ND (not detected at or above the limit of quantitation of 0.00561 mg a.i./L, control), 1.06, 2.01, 3.46, 5.67, 9.70 mg a.i./L. Mean measured concentrations of 2-methyl-4-isothiazolin-3-one ranged from 82 to 97% of nominal concentrations.
Exposure of the rainbow trout, Oncorhynchus mykiss, to 2-methyl-4-isothiazolin-3-one.Technical resulted in a 96-hour median lethal concentration (LC50) of 4.77 mg a.i./L, with a 95% confidence interval of 4.21 to 5.42 mg a.i./L. The 96-hour no-observed effect concentration (NOEC) for 2-methyl-4-isothiazolin-3-one was 2.01 mg a.i. /L and the slope of the concentration response curve is 9.4.
The results of the key study indicated that exposure of the rainbow trout, Oncorhynchus mykiss, to 2-methyl-4-isothiazolin-3-one.Technical resulted in a 96-hour median lethal concentration (LC50) of 4.77 mg a.i./L, with a 95% confidence interval of 4.21 to 5.42 mg a.i./L.
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