Fatty acids C16-18 (even numbered), mono, di and tri esters with sucrose

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Feb 24 - June 19 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.1075 (Freshwater and Saltwater Fish Acute Toxicity Test)

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was ground, and added to a weighed beaker. The test substance was then added to 13 L of dilution water. The solution was then mixed for 48 hrs, and then allowed to settle for 5 hrs.
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): Precipitate was present in the dilution solution. Great care was taken when decanting the dilution water into the test chamber to avoid transferring precipitate. No evidence of precipitate was seen in the test chambers.

Test organisms (species):

Pimephales promelas

Details on test organisms:

TEST ORGANISM
- Common name: fathead minnow
- Source: Chesapeake Cultures, Inc., Hayes, Virginia, USA
- Age at study initiation (mean and range, SD): juveniles
- Length at study initiation (length definition, mean, range and SD): 2.4-2.8 cm, average 2.6 cm (control fish at end of study)
- Weight at study initiation (mean and range, SD): 0.09-0.15 g, average 0.12 g (control fish at end of study)

ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions (same as test or not): same
- Type and amount of food during acclimation: brine shrimp nauplii
- Feeding frequency during acclimation: daily, except for two days prior to the test

FEEDING DURING TEST
- Food type: brine shrimp nauplii
- Frequency: Daily, except for two days prior to the test

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

148 mg/L as CaCO3

Test temperature:

22 +/- 1 °C

pH:

7.9-8.3

Dissolved oxygen:

>= 6.2 mg/L

Conductivity:

355 µS/cm

Nominal and measured concentrations:

Nominal: control, 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 25 L
- Material, size, headspace, fill volume: stainless steel, 25 L size filled to 12 L
- No. of organisms per vessel: 7
- Biomass loading rate: 0.063 g fish/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: 40 m deep well on laboratory site
- Total organic carbon: 2.0-5.2 mg C/L
- Metals: No metals were present above the detection limits.
- Pesticides: No pesticides were present above the detection limits.
- Chlorine: 4.3 mg/L
- Alkalinity: 180 mg/L as CaCO3
- Ca/mg ratio: 2.6

OTHER TEST CONDITIONS
- Photoperiod: 16 hrs light/8 hrs darkness with 30 min transition period
- Light intensity: 350 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mortality, abnormal behaviour with observation intervals at 18, 24, 48, 72, and 96 hrs after start of exposure

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LL50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

WAF

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: None
- Mortality of control: None
- Abnormal responses: None

Sublethal observations / clinical signs:

Results

Observation	Mortality - Control Group	Mortality - 100 mg/L Group
18 hrs	0	0
24 hrs	0	0
48 hrs	0	0
72 hrs	0	0
96 hrs	0	0

Validity criteria fulfilled:

yes

Conclusions:

The 96-hr LL50 in fathead minnow is > 100 mg/L (WAF).

Executive summary:

The acute toxicity of sucrose esters to fish was determined in an OECD Guideline 203 study. Groups of seven fish were exposed to 0 or 100 mg/L of sucrose esters for 96 hrs. Due to the complex nature of the test substance, results were reported as water accomodated fraction (WAF). No fish in the test or control groups died or showed abnormal behaviour. The 96-hr LL50 is therefore >100 mg/L (WAF).

Description of key information

LL50 (96 h) > 100 mg/L (nominal, OECD 203)

Additional information

One experimental study is available investigating the toxicity of the test item to the freshwater fish Pimephales promelas. The study was performed according to OECD 203 (GLP) under static conditions. Due to the low water solubility the test solutions were prepared as Water Accommodated Fractions (WAF) and the loading rate of 100 mg/L (nominal) was tested. The WAF was prepared by adding an appropriate amount of test item to test medium followed by mixing for 48 h and a settling time of 5 h. Since undissolved particles were present in the solution it was carefully decanted to the exposure vessels in order to avoid undissolved material in the test vessels. No evidence of precipitate was seen in the test chambers. After exposure of 96 h no mortality was recorded resulting in a LL50 (96 h) of > 100 mg/L based on the nominal loading rate.