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Biodegradation in water: screening tests

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biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
16 Mar - 13 Apr 1993
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
according to guideline
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
GLP compliance:
Oxygen conditions:
Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Details on inoculum:
Sampling date: 1993-03-15
Duration of test (contact time):
28 d
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
The substance was suspended in mineral medium, inoculated with activated sludge and incubated for 28 d under aerobic conditions in the dark at 19-21°C. The sludge concentration of 30 mg suspended particles/L was used.

ThoD of the test substance: 1665 mg O2/g test material
Reference substance:
Test performance:
No degradation observed within 28 d
Key result
% degradation (O2 consumption)
St. dev.:
Sampling time:
28 d
Details on results:
Points of degradation plot (test substance):
0 % degradation after 2 d
0 % degradation after 6 d
0 % degradation after 8 d
0 % degradation after 10 d
0 % degradation after 14 d
0 % degradation after 16 d
0 % degradation after 20 d
0 % degradation after 22 d
0 % degradation after 28 d
Results with reference substance:
Points of degradation plot (reference substance):
0 % degradation after 2 d
56 % degradation after 6 d
68 % degradation after 8 d
72 % degradation after 10 d
74 % degradation after 14 d
71 % degradation after 16 d
71 % degradation after 20 d
70 % degradation after 22 d
70 % degradation after 28 d
Validity criteria fulfilled:
Interpretation of results:
under test conditions no biodegradation observed
The test item was found to be not readily biodegradable based on O2 consumption.

Description of key information

The structural analogue substance was not readily biodegradable in an OECD 301F test (0% based on O2 consumption). Disperse Yellow 126 is not considered to be readily biodegradable either.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

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